TY - JOUR
T1 - A generalized approach to thermodynamic properties of biomolecules for use in bioseparation process design
AU - Ahamed, Tangir
AU - Ottens, Marcel
AU - Nfor, Beckley K.
AU - Van Dedem, Gijs W.K.
AU - Van Der Wielen, Luuk A.M.
PY - 2006/3/15
Y1 - 2006/3/15
N2 - Bioseparation techniques exploit the differences of physicochemical or thermodynamic properties between the product and the contaminants. Rapid development of a downstream process, therefore, requires physicochemical and thermodynamic characterization of the components to be separated. In this paper, we investigate whether a generalized thermodynamic interrelation exists among different parameters. For instance activity coefficients, osmotic virial coefficients and the solubility of macromolecules are interrelated to each other. Experimental determination of any one of these parameters can be translated across the boundaries of different separation techniques. A number of downstream separation processes, including size-exclusion chromatography, hydrophobic-interaction chromatography, reversed-phase chromatography, aqueous two-phase separation, crystallization and precipitation, are found to be explained and designed using this generalized thermodynamics. This generalization of thermodynamic properties together with high-throughput experimentation provides a systematic and high-speed approach to bioseparation process development and optimization. The applicability of this approach for bioseparation process design was investigated by a case study on nystatin, a medium-sized biomolecule. The distribution coefficients of nystatin in reversed-phase chromatography showed straightforward relationship with the solubilities at various solvent compositions and the experimental data supported the trend of the relationship.
AB - Bioseparation techniques exploit the differences of physicochemical or thermodynamic properties between the product and the contaminants. Rapid development of a downstream process, therefore, requires physicochemical and thermodynamic characterization of the components to be separated. In this paper, we investigate whether a generalized thermodynamic interrelation exists among different parameters. For instance activity coefficients, osmotic virial coefficients and the solubility of macromolecules are interrelated to each other. Experimental determination of any one of these parameters can be translated across the boundaries of different separation techniques. A number of downstream separation processes, including size-exclusion chromatography, hydrophobic-interaction chromatography, reversed-phase chromatography, aqueous two-phase separation, crystallization and precipitation, are found to be explained and designed using this generalized thermodynamics. This generalization of thermodynamic properties together with high-throughput experimentation provides a systematic and high-speed approach to bioseparation process development and optimization. The applicability of this approach for bioseparation process design was investigated by a case study on nystatin, a medium-sized biomolecule. The distribution coefficients of nystatin in reversed-phase chromatography showed straightforward relationship with the solubilities at various solvent compositions and the experimental data supported the trend of the relationship.
KW - Activity coefficient
KW - Biomolecule
KW - Bioprocess design
KW - Bioseparation
KW - Nystatin
KW - Osmotic virial coefficient
UR - http://www.scopus.com/inward/record.url?scp=33644748090&partnerID=8YFLogxK
U2 - 10.1016/j.fluid.2005.12.011
DO - 10.1016/j.fluid.2005.12.011
M3 - Article
AN - SCOPUS:33644748090
SN - 0378-3812
VL - 241
SP - 268
EP - 282
JO - Fluid Phase Equilibria
JF - Fluid Phase Equilibria
IS - 1-2
ER -