TY - GEN
T1 - A gravity driven microfluidic platform for DNA enrichment
AU - McCarthy, Conor E.
AU - Dalton, Tara
AU - Davies, Mark
PY - 2013
Y1 - 2013
N2 - There is currently considerable interest in the development of microfluidic based lab-on-chip devices for sample preparation in next generation sequencing. One of these steps is DNA enrichment which often relies on conventional PCR to amplify the sample to detectable levels. To successfully automate this step, technologies are required whereby the samples are selectively inputted, thermocycled and selectively dispensed, all non invasively and therefore leading to no contamination issues. In this study such a system was created through the use of liquid-liquid plugs flowing in a gravity driven siphon. Plug generation was achieved through an innovative approach whereby a hydrophobic tube was traversed between two immiscible fluids (silicon oil and PCR reagents) and successful amplification was shown for Beta-2-Microfloblin (B2M).
AB - There is currently considerable interest in the development of microfluidic based lab-on-chip devices for sample preparation in next generation sequencing. One of these steps is DNA enrichment which often relies on conventional PCR to amplify the sample to detectable levels. To successfully automate this step, technologies are required whereby the samples are selectively inputted, thermocycled and selectively dispensed, all non invasively and therefore leading to no contamination issues. In this study such a system was created through the use of liquid-liquid plugs flowing in a gravity driven siphon. Plug generation was achieved through an innovative approach whereby a hydrophobic tube was traversed between two immiscible fluids (silicon oil and PCR reagents) and successful amplification was shown for Beta-2-Microfloblin (B2M).
UR - http://www.scopus.com/inward/record.url?scp=84903447420&partnerID=8YFLogxK
U2 - 10.1115/IMECE2013-64599
DO - 10.1115/IMECE2013-64599
M3 - Conference contribution
AN - SCOPUS:84903447420
SN - 9780791856321
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
BT - Fluids Engineering Systems and Technologies
PB - American Society of Mechanical Engineers (ASME)
T2 - ASME 2013 International Mechanical Engineering Congress and Exposition, IMECE 2013
Y2 - 15 November 2013 through 21 November 2013
ER -