@inproceedings{fc340a76d4bf42e6a5bc1c778895a070,
title = "A multi-modal multi-photon microscope for biological imaging",
abstract = "We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO 4 laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.",
keywords = "CARS, Drug delivery, Live cells, Multiphoton microscopy, NLO spectroscopy, SHG, Two photon fluorescence",
author = "Rabah Mouras and Andrew Downes and Grigore Rischitor and Meropi Mari and Alistair Elfick",
year = "2010",
doi = "10.1117/12.844236",
language = "English",
isbn = "9780819479655",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences X",
note = "Multiphoton Microscopy in the Biomedical Sciences X ; Conference date: 24-01-2010 Through 26-01-2010",
}
