Abstract
We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO 4 laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.
| Original language | English |
|---|---|
| Title of host publication | Multiphoton Microscopy in the Biomedical Sciences X |
| DOIs | |
| Publication status | Published - 2010 |
| Externally published | Yes |
| Event | Multiphoton Microscopy in the Biomedical Sciences X - San Francisco, CA, United States Duration: 24 Jan 2010 → 26 Jan 2010 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 7569 |
| ISSN (Print) | 1605-7422 |
Conference
| Conference | Multiphoton Microscopy in the Biomedical Sciences X |
|---|---|
| Country/Territory | United States |
| City | San Francisco, CA |
| Period | 24/01/10 → 26/01/10 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- CARS
- Drug delivery
- Live cells
- Multiphoton microscopy
- NLO spectroscopy
- SHG
- Two photon fluorescence
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