TY - JOUR
T1 - A novel ICE in the genome of Shewanella putrefaciens W3-18-1
T2 - Comparison with the SXT/R391 ICE-like elements
AU - Pembroke, J. Tony
AU - Piterina, Anna V.
PY - 2006/11
Y1 - 2006/11
N2 - A novel R391-like ICE (integrating conjugative element) has been detected in the 4.2 MB genome of Shewanella putrefaciens W3-18-1 located on three different contigs. Assembly of the ICE encoding contigs based on similarity with R391 revealed a mosaic element of plasmid, phage and transposon-like sequences typical of SXT/R391 ICE-like elements. The element, which is 110 057 bp in length, was highly similar to R391 sequences, with most related ORFs showing >96% amino acid sequence identity. The element, designated ICESpuPO1, contained a number of inserts determining resistance to copper and other heavy metals and a broad-spectrum RND efflux pump similar to antibiotic efflux systems. The element was integrated into the Shewanella prfC gene in a manner similar to related ICE-like elements. The chromosomal element junctions contained a 17-bp SXT/R391-like attL and attR site and an unannotated ORF between attL and the ICE integrase encoding a putative recombinational directional factor necessary for excision, with 100% amino acid identity to the R391 ORF4 product.
AB - A novel R391-like ICE (integrating conjugative element) has been detected in the 4.2 MB genome of Shewanella putrefaciens W3-18-1 located on three different contigs. Assembly of the ICE encoding contigs based on similarity with R391 revealed a mosaic element of plasmid, phage and transposon-like sequences typical of SXT/R391 ICE-like elements. The element, which is 110 057 bp in length, was highly similar to R391 sequences, with most related ORFs showing >96% amino acid sequence identity. The element, designated ICESpuPO1, contained a number of inserts determining resistance to copper and other heavy metals and a broad-spectrum RND efflux pump similar to antibiotic efflux systems. The element was integrated into the Shewanella prfC gene in a manner similar to related ICE-like elements. The chromosomal element junctions contained a 17-bp SXT/R391-like attL and attR site and an unannotated ORF between attL and the ICE integrase encoding a putative recombinational directional factor necessary for excision, with 100% amino acid identity to the R391 ORF4 product.
KW - ICESpuPO1
KW - Mobile efflux pumps
KW - Shewanella genome
KW - SXT/R391-like ICEs
UR - http://www.scopus.com/inward/record.url?scp=33749430782&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2006.00452.x
DO - 10.1111/j.1574-6968.2006.00452.x
M3 - Article
C2 - 17020552
AN - SCOPUS:33749430782
SN - 0378-1097
VL - 264
SP - 80
EP - 88
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -