TY - JOUR
T1 - A possible influence of extracellular polysaccharides on the analysis of intracellular metabolites from Trichoderma harzianum grown under carbon-limited conditions
AU - Gelain, Lucas
AU - Geraldo da Cruz Pradella, José
AU - Carvalho da Costa, Aline
AU - van der Wielen, Luuk
AU - van Gulik, Walter M.
N1 - Publisher Copyright:
© 2020 The Author(s)
PY - 2021/5
Y1 - 2021/5
N2 - Intracellular metabolites were evaluated during the continuous growth of Trichoderma harzianum P49P11 under carbon-limited conditions. Four different conditions in duplicate were investigated (10 and 20 g/L of glucose, 5.26/5.26 g/L of fructose/glucose and 10 g/L of sucrose in the feed). Differences in the values of some specific concentrations of intracellular metabolites were observed at steady-state for the duplicates. The presence of extracellular polysaccharide was confirmed in the supernatant of all conditions based on FT-IR and proton NMR. Fragments of polysaccharides from the cell wall could be released due to the shear stress and since the cells can consume them under carbon-limited conditions, this could create an unpredictable carbon flow rate into the cells. According to the values of the metabolite concentrations, it was considered that the consumption of those fragments was interfering with the analysis.
AB - Intracellular metabolites were evaluated during the continuous growth of Trichoderma harzianum P49P11 under carbon-limited conditions. Four different conditions in duplicate were investigated (10 and 20 g/L of glucose, 5.26/5.26 g/L of fructose/glucose and 10 g/L of sucrose in the feed). Differences in the values of some specific concentrations of intracellular metabolites were observed at steady-state for the duplicates. The presence of extracellular polysaccharide was confirmed in the supernatant of all conditions based on FT-IR and proton NMR. Fragments of polysaccharides from the cell wall could be released due to the shear stress and since the cells can consume them under carbon-limited conditions, this could create an unpredictable carbon flow rate into the cells. According to the values of the metabolite concentrations, it was considered that the consumption of those fragments was interfering with the analysis.
KW - Carbon limitation
KW - Continuous culture
KW - Fragments from extracellular polysaccharides
KW - Metabolites
UR - http://www.scopus.com/inward/record.url?scp=85097878241&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2020.12.002
DO - 10.1016/j.funbio.2020.12.002
M3 - Article
C2 - 33910678
AN - SCOPUS:85097878241
SN - 1878-6146
VL - 125
SP - 368
EP - 377
JO - Fungal Biology
JF - Fungal Biology
IS - 5
ER -