A quantitative free convection DNA amplifier

John Daly, Mark Davies

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The Polymerase Chain Reaction (PCR) has been used extensively to amplify targeted nucleic acids for many applications in molecular biology and, increasingly, in medical diagnostics. Outlined in this paper is a PCR device which takes account of the advantages offered by free convection. The design is, in it fundamental format a time-wise isothermal well-based thermocycler. A temperature gradient induced across the well causes convection forces to circulate the sample through the required temperatures necessary for amplification. Quantitative amplification is demonstrated with real time measurements of SYBR Green I fluorescence within the free convective DNA amplifier. Amplification of an 86-bp fragment of the pGEM®-T vector (Promega) is performed in a 25μl volume in eight minutes. A 10-fold dilution series and methods for calculating effective cycle times are presented. Also detailed within this paper are PIV and thermal imaging results of the free convection cavity. This device presents an opportunity for the development of a practical and inexpensive gene-expression measurement system.

Original languageEnglish
Title of host publication2007 Proceedings of the ASME/JSME Thermal Engineering Summer Heat Transfer Conference - HT 2007
Pages41-50
Number of pages10
DOIs
Publication statusPublished - 2007
Event2007 ASME/JSME Thermal Engineering Summer Heat Transfer Conference, HT 2007 - Vancouver, BC, Canada
Duration: 8 Jul 200712 Jul 2007

Publication series

Name2007 Proceedings of the ASME/JSME Thermal Engineering Summer Heat Transfer Conference - HT 2007
Volume3

Conference

Conference2007 ASME/JSME Thermal Engineering Summer Heat Transfer Conference, HT 2007
Country/TerritoryCanada
CityVancouver, BC
Period8/07/0712/07/07

Fingerprint

Dive into the research topics of 'A quantitative free convection DNA amplifier'. Together they form a unique fingerprint.

Cite this