A SIMPLIFIED PROCEDURE FOR ELIMINATING THE NEGATIVE INTERFERENCE OF BILIRUBIN IN THE JAFFE REACTION FOR CREATININE

J Tony Pembroke, N. O'Leary, P. F. Duggan

Research output: Contribution to journalArticlepeer-review

Abstract

The negative interference by bilirubin in the measurement of creatinine by the Jaffé procedure is well documented. We describe a procedure for the elimination of interfering bilirubin by oxidation to biliverdin through the addition of potassium ferricyanide before the alkaline picrate reagent. We detected no negative interference with the addition of as much as 600 μmol/L bilirubin. The procedure shows good agreement with a recently described blank rate method for analyzing icteric samples.

Original languageEnglish (Ireland)
Pages (from-to)1749-1751
Number of pages3
JournalClinical Chemistry
Volume38
Issue number9
Publication statusPublished - 1992

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