ADAPTING THE URICASE PEROXIDASE PROCEDURE FOR PLASMA URATE TO REDUCE INTERFERENCE DUE TO HEMOLYSIS, ICTERUS OR LIPEMIA

J Tony Pembroke; N. O. Leary; P. F. Duggan

doi:10.1177/000456329202900113

ADAPTING THE URICASE PEROXIDASE PROCEDURE FOR PLASMA URATE TO REDUCE INTERFERENCE DUE TO HEMOLYSIS, ICTERUS OR LIPEMIA

J Tony Pembroke
, N. O. Leary
, P. F. Duggan

Retired Staff

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

The estimation of serum urate by uricase/peroxidase procedures involving single point bichromatic absorbance monitoring gives inaccurate results with samples which are haemolysed, icteric or lipaemic. These inaccuracies can be greatly minimized by reducing the rate of chromogen formation and applying the assay in a kinetic mode.

Original language	English (Ireland)
Pages (from-to)	85-89
Number of pages	5
Journal	Annals of Clinical Biochemistry
Volume	29
Issue number	1
DOIs	https://doi.org/10.1177/000456329202900113
Publication status	Published - 1992

Keywords

Chromophoric interference
Kinetic procedure
Reduced uricase

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10.1177/000456329202900113

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title = "ADAPTING THE URICASE PEROXIDASE PROCEDURE FOR PLASMA URATE TO REDUCE INTERFERENCE DUE TO HEMOLYSIS, ICTERUS OR LIPEMIA",

abstract = "The estimation of serum urate by uricase/peroxidase procedures involving single point bichromatic absorbance monitoring gives inaccurate results with samples which are haemolysed, icteric or lipaemic. These inaccuracies can be greatly minimized by reducing the rate of chromogen formation and applying the assay in a kinetic mode.",

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AU - Duggan, P. F.

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AB - The estimation of serum urate by uricase/peroxidase procedures involving single point bichromatic absorbance monitoring gives inaccurate results with samples which are haemolysed, icteric or lipaemic. These inaccuracies can be greatly minimized by reducing the rate of chromogen formation and applying the assay in a kinetic mode.

KW - Chromophoric interference

KW - Kinetic procedure

KW - Reduced uricase

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ADAPTING THE URICASE PEROXIDASE PROCEDURE FOR PLASMA URATE TO REDUCE INTERFERENCE DUE TO HEMOLYSIS, ICTERUS OR LIPEMIA

Abstract

Keywords

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