Adsorption and activity of a domoic acid binding antibody fragment on mesoporous silicates

Xuejun Hu; Stefania Spada; Simon White; Sarah Hudson; Edmond Magner; J. Gerard Wall

doi:10.1021/jp062423e

Adsorption and activity of a domoic acid binding antibody fragment on mesoporous silicates

Xuejun Hu, Stefania Spada, Simon White, Sarah Hudson, Edmond Magner, J. Gerard Wall

Department of Chemical Sciences

University of Limerick

Research output: Contribution to journal › Article › peer-review

Abstract

The adsorption of an anti-domoic acid single-chain Fv (scFv) antibody fragment onto a range of mesoporous silicate supports was investigated. The scPv fragment adsorbed to all materials investigated, and pI had an apparently large effect on coating, with the greatest - and fastest - adsorption found on the most negatively charged silicates. Maximal coating levels attainable did not reflect the pore diameters of the materials. The immobilized antibody was functional on all materials and bound its antigen, a naturally occurring neurotoxin produced by shellfish, in a rapidly saturating manner that suggested the antibody adsorbed in a multilayer on the mesoporous particles. The antigen:antibody ratio decreased from 1:1.3 to <1:10 with increasing concentration of immobilized antibody, and the immobilized scFv exhibited no detectable reduction in domoic acid binding over a 42-day incubation period.

Original language	English
Pages (from-to)	18703-18709
Number of pages	7
Journal	Journal of Physical Chemistry B
Volume	110
Issue number	37
DOIs	https://doi.org/10.1021/jp062423e
Publication status	Published - 21 Sep 2006

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title = "Adsorption and activity of a domoic acid binding antibody fragment on mesoporous silicates",

abstract = "The adsorption of an anti-domoic acid single-chain Fv (scFv) antibody fragment onto a range of mesoporous silicate supports was investigated. The scPv fragment adsorbed to all materials investigated, and pI had an apparently large effect on coating, with the greatest - and fastest - adsorption found on the most negatively charged silicates. Maximal coating levels attainable did not reflect the pore diameters of the materials. The immobilized antibody was functional on all materials and bound its antigen, a naturally occurring neurotoxin produced by shellfish, in a rapidly saturating manner that suggested the antibody adsorbed in a multilayer on the mesoporous particles. The antigen:antibody ratio decreased from 1:1.3 to <1:10 with increasing concentration of immobilized antibody, and the immobilized scFv exhibited no detectable reduction in domoic acid binding over a 42-day incubation period.",

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T1 - Adsorption and activity of a domoic acid binding antibody fragment on mesoporous silicates

AU - Hu, Xuejun

AU - Spada, Stefania

AU - White, Simon

AU - Hudson, Sarah

AU - Magner, Edmond

AU - Wall, J. Gerard

PY - 2006/9/21

Y1 - 2006/9/21

N2 - The adsorption of an anti-domoic acid single-chain Fv (scFv) antibody fragment onto a range of mesoporous silicate supports was investigated. The scPv fragment adsorbed to all materials investigated, and pI had an apparently large effect on coating, with the greatest - and fastest - adsorption found on the most negatively charged silicates. Maximal coating levels attainable did not reflect the pore diameters of the materials. The immobilized antibody was functional on all materials and bound its antigen, a naturally occurring neurotoxin produced by shellfish, in a rapidly saturating manner that suggested the antibody adsorbed in a multilayer on the mesoporous particles. The antigen:antibody ratio decreased from 1:1.3 to <1:10 with increasing concentration of immobilized antibody, and the immobilized scFv exhibited no detectable reduction in domoic acid binding over a 42-day incubation period.

AB - The adsorption of an anti-domoic acid single-chain Fv (scFv) antibody fragment onto a range of mesoporous silicate supports was investigated. The scPv fragment adsorbed to all materials investigated, and pI had an apparently large effect on coating, with the greatest - and fastest - adsorption found on the most negatively charged silicates. Maximal coating levels attainable did not reflect the pore diameters of the materials. The immobilized antibody was functional on all materials and bound its antigen, a naturally occurring neurotoxin produced by shellfish, in a rapidly saturating manner that suggested the antibody adsorbed in a multilayer on the mesoporous particles. The antigen:antibody ratio decreased from 1:1.3 to <1:10 with increasing concentration of immobilized antibody, and the immobilized scFv exhibited no detectable reduction in domoic acid binding over a 42-day incubation period.

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Adsorption and activity of a domoic acid binding antibody fragment on mesoporous silicates

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