Ameliorative effects of aqueous extract from rosemary on oxidative stress and inflammation pathways caused by a high-fat diet in C57BL/6 mice

Ana Mara de Oliveira E Silva, Raquel Oliveira Pereira, Anne Karoline de Souza Oliveira, Fernanda Santana Harris, Illana Louise Pereira de Melo, Thiago Henrique Almeida-Souza, Luciana Tedesco Yoshime, Caroline Dos Santos Melo, Jymmys Lopes Dos Santos, Elma Regina Silva de Andrade-Wartha, Bruno Cogliati, Daniel Granato, Jorge Mancini-Filho

Research output: Contribution to journalArticlepeer-review

Abstract

Rosemary is an herb exhibits biological properties, attenuates inflammation, oxidative stress, and improves lipid profile. Here, we evaluated the effects of rosemary aqueous extract (RE) on mice fed with a high-fat diet (HFD). Male C57BL/6 mice were administered a control diet or HFD for 10 weeks. The treated groups received RE in the diet at different concentrations: 25, 250, and 500 mg/100 g. After 10 weeks, serum concentrations of glucose, lipid, insulin, leptin, adiponectin, and cytokines were evaluated and the oxygen radical absorbance capacity was determined. Histological analysis was performed to determine the concentrations of triacylglycerides (TG), total cholesterol, cytokines, and antioxidant enzymes as well as the expression of genes involved in lipid metabolism, oxidative stress, and inflammation. The dietary RE ameliorated HFD-induced weight gain, adipose tissue weight, glucose intolerance, and insulin, leptin, and free fatty acid levels. Reduction in hepatic TG deposition was observed. The levels of inflammatory cytokines decreased, and the expression of genes involved in lipid metabolism increased. RE mitigated oxidative stress and reduced the production of reactive oxygen species in HepG2 and 3T3-L1 cells. Therefore, RE is a potential therapeutic agent for the prevention of inflammation and oxidative stress outcomes associated with obesity.

Original languageEnglish
Pages (from-to)459-472
Number of pages14
JournalApplied Physiology, Nutrition and Metabolism
Volume49
Issue number4
DOIs
Publication statusPublished - 1 Apr 2024

Keywords

  • inflammation
  • metabolic syndrome
  • obesity
  • oxidative stress
  • phenolic compounds
  • rosemary

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