TY - JOUR
T1 - Ameliorative effects of aqueous extract from rosemary on oxidative stress and inflammation pathways caused by a high-fat diet in C57BL/6 mice
AU - de Oliveira E Silva, Ana Mara
AU - Pereira, Raquel Oliveira
AU - Oliveira, Anne Karoline de Souza
AU - Harris, Fernanda Santana
AU - de Melo, Illana Louise Pereira
AU - Almeida-Souza, Thiago Henrique
AU - Yoshime, Luciana Tedesco
AU - Dos Santos Melo, Caroline
AU - Lopes Dos Santos, Jymmys
AU - de Andrade-Wartha, Elma Regina Silva
AU - Cogliati, Bruno
AU - Granato, Daniel
AU - Mancini-Filho, Jorge
N1 - Publisher Copyright:
© 2024 The Author(s).
PY - 2024/4/1
Y1 - 2024/4/1
N2 - Rosemary is an herb exhibits biological properties, attenuates inflammation, oxidative stress, and improves lipid profile. Here, we evaluated the effects of rosemary aqueous extract (RE) on mice fed with a high-fat diet (HFD). Male C57BL/6 mice were administered a control diet or HFD for 10 weeks. The treated groups received RE in the diet at different concentrations: 25, 250, and 500 mg/100 g. After 10 weeks, serum concentrations of glucose, lipid, insulin, leptin, adiponectin, and cytokines were evaluated and the oxygen radical absorbance capacity was determined. Histological analysis was performed to determine the concentrations of triacylglycerides (TG), total cholesterol, cytokines, and antioxidant enzymes as well as the expression of genes involved in lipid metabolism, oxidative stress, and inflammation. The dietary RE ameliorated HFD-induced weight gain, adipose tissue weight, glucose intolerance, and insulin, leptin, and free fatty acid levels. Reduction in hepatic TG deposition was observed. The levels of inflammatory cytokines decreased, and the expression of genes involved in lipid metabolism increased. RE mitigated oxidative stress and reduced the production of reactive oxygen species in HepG2 and 3T3-L1 cells. Therefore, RE is a potential therapeutic agent for the prevention of inflammation and oxidative stress outcomes associated with obesity.
AB - Rosemary is an herb exhibits biological properties, attenuates inflammation, oxidative stress, and improves lipid profile. Here, we evaluated the effects of rosemary aqueous extract (RE) on mice fed with a high-fat diet (HFD). Male C57BL/6 mice were administered a control diet or HFD for 10 weeks. The treated groups received RE in the diet at different concentrations: 25, 250, and 500 mg/100 g. After 10 weeks, serum concentrations of glucose, lipid, insulin, leptin, adiponectin, and cytokines were evaluated and the oxygen radical absorbance capacity was determined. Histological analysis was performed to determine the concentrations of triacylglycerides (TG), total cholesterol, cytokines, and antioxidant enzymes as well as the expression of genes involved in lipid metabolism, oxidative stress, and inflammation. The dietary RE ameliorated HFD-induced weight gain, adipose tissue weight, glucose intolerance, and insulin, leptin, and free fatty acid levels. Reduction in hepatic TG deposition was observed. The levels of inflammatory cytokines decreased, and the expression of genes involved in lipid metabolism increased. RE mitigated oxidative stress and reduced the production of reactive oxygen species in HepG2 and 3T3-L1 cells. Therefore, RE is a potential therapeutic agent for the prevention of inflammation and oxidative stress outcomes associated with obesity.
KW - inflammation
KW - metabolic syndrome
KW - obesity
KW - oxidative stress
KW - phenolic compounds
KW - rosemary
UR - http://www.scopus.com/inward/record.url?scp=85189751788&partnerID=8YFLogxK
U2 - 10.1139/apnm-2023-0157
DO - 10.1139/apnm-2023-0157
M3 - Article
C2 - 38048548
AN - SCOPUS:85189751788
SN - 1715-5312
VL - 49
SP - 459
EP - 472
JO - Applied Physiology, Nutrition and Metabolism
JF - Applied Physiology, Nutrition and Metabolism
IS - 4
ER -