TY - JOUR
T1 - An electrochemical NADH biosensor based on a nanoporous gold electrode modified with diaphorase and an osmium polymer
AU - Otero, Fernando
AU - Mandal, Tanushree
AU - Leech, Dónal
AU - Magner, Edmond
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/11
Y1 - 2022/11
N2 - NADH is a cofactor used by a wide range of dehydrogenases. Measurement of the concentration of NADH is widely used to measure the activity of enzymes. Extensive efforts have been made to develop electrochemical sensors for NADH determination at low overpotentials to avoid interferences. The development of an enzymatic electrochemical biosensor for the detection of NADH is described. Nanoporous gold electrodes were utilised for the immobilization of diaphorase and osmium-based polymer Os(bpy)2(PVI). Nanoporous gold electrodes of different pore sizes were manufactured by varying the dealloying temperature. To optimise the enzymatic response, the concentrations of polymer and enzyme, average pore size and the operating temperature were examined with the optimal performance observed using 10 µL of Os(bpy)2(PVI) and 5 µL of diaphorase at concentrations of 6 and 10 mg/mL, respectively, on nanoporous gold electrodes with an average pore size of 5.9 nm. The biosensor showed a high sensitivity of 89.6 µA/cm2 mM, a low LOD of 0.8 µM and a linear range from 5 to 100 µM at a potential of 0.35 V vs Ag/AgCl at a temperature of 40 °C.
AB - NADH is a cofactor used by a wide range of dehydrogenases. Measurement of the concentration of NADH is widely used to measure the activity of enzymes. Extensive efforts have been made to develop electrochemical sensors for NADH determination at low overpotentials to avoid interferences. The development of an enzymatic electrochemical biosensor for the detection of NADH is described. Nanoporous gold electrodes were utilised for the immobilization of diaphorase and osmium-based polymer Os(bpy)2(PVI). Nanoporous gold electrodes of different pore sizes were manufactured by varying the dealloying temperature. To optimise the enzymatic response, the concentrations of polymer and enzyme, average pore size and the operating temperature were examined with the optimal performance observed using 10 µL of Os(bpy)2(PVI) and 5 µL of diaphorase at concentrations of 6 and 10 mg/mL, respectively, on nanoporous gold electrodes with an average pore size of 5.9 nm. The biosensor showed a high sensitivity of 89.6 µA/cm2 mM, a low LOD of 0.8 µM and a linear range from 5 to 100 µM at a potential of 0.35 V vs Ag/AgCl at a temperature of 40 °C.
KW - Diaphorase
KW - Electrochemical biosensor
KW - Enzymatic biosensor
KW - NADH
KW - Nanoporous gold electrode
KW - Osmium-based polymer
UR - http://www.scopus.com/inward/record.url?scp=85137738592&partnerID=8YFLogxK
U2 - 10.1016/j.snr.2022.100117
DO - 10.1016/j.snr.2022.100117
M3 - Article
AN - SCOPUS:85137738592
SN - 2666-0539
VL - 4
JO - Sensors and Actuators Reports
JF - Sensors and Actuators Reports
M1 - 100117
ER -