Antibacterial and antioxidative activity of O-amine functionalized chitosan

Tamer M. Tamer, Mohamed A. Hassan, Ahmed M. Omer, Katarína Valachová, Mohamed S.Mohy Eldin, Maurice N. Collins, Ladislav Šoltés

Research output: Contribution to journalArticlepeer-review

Abstract

Cinnamaldehyde was immobilized to O-amine functionalized chitosan via a coupling reaction. Fourier transform infrared spectroscopy confirmed N-cinnamyl substitution. Wetting analyses demonstrate more hydrophobicity in the N-cinnamyl substituted O-amine functionalized chitosan compared to chitosan or unsubstituted O-amine functionalized chitosan. Thermal gravimetric analysis and differential scanning calorimetry demonstrates that the prepared N-cinnamyl substituted O-amine functionalized chitosan exhibits higher thermostability than unmodified chitosan at temperatures in which polysaccharides are commonly stored and utilised. The N-cinnamyl substituted O-amine functionalized chitosan, against four different bacteria strains [two gram-positive (Staphylococcus aureus and Bacillus cereus) and two gram-negative (Escherichia coli and Pseudomonas aeruginosa)], displays promotion of inhibition activity against these bacterial strains. Finally, the antioxidative activity of the N-cinnamyl substituted O-amine functionalized chitosan was compared with those activities of chitosan and O-amine functionalized chitosan. This was evaluated by uninhibited and inhibited hyaluronan degradation and ABTS assay. The N-cinnamyl substituted O-amine functionalized chitosan shows a lower activity towards donating a hydrogen radical compared to chitosan or O-amine functionalized chitosan. On the other hand, the N-cinnamyl substituted O-amine functionalized chitosan exhibited a higher ability to scavenge the ABTS[rad]+ cation radical compared to chitosan and O-amine functionalized chitosan.

Original languageEnglish
Pages (from-to)441-450
Number of pages10
JournalCarbohydrate Polymers
Volume169
DOIs
Publication statusPublished - 1 Aug 2017

Keywords

  • Bacillus cereus
  • Chitosan derivatives
  • Escherichia coli
  • Pseudomonas aeruginosa
  • Staphylococcus aureus

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