TY - JOUR
T1 - Antibodies@MOFs
T2 - An In Vitro Protective Coating for Preparation and Storage of Biopharmaceuticals
AU - Feng, Yifan
AU - Wang, Huanrong
AU - Zhang, Sainan
AU - Zhao, Yu
AU - Gao, Jia
AU - Zheng, Yunyi
AU - Zhao, Peng
AU - Zhang, Zhenjie
AU - Zaworotko, Michael J.
AU - Cheng, Peng
AU - Ma, Shengqian
AU - Chen, Yao
N1 - Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/1/11
Y1 - 2019/1/11
N2 - Antibodies have emerged as a fast-growing category of biopharmaceuticals that have been widely applied in scientific research, medical diagnosis, and disease treatment. However, many antibodies and other biopharmaceuticals display inferior biophysical properties, such as low stability and a propensity to undergo aggregation. Enhancing the stability of biopharmaceuticals is essential for their wide applications. Here, a facile in vitro protective coating strategy based on metal–organic frameworks (MOFs) is proposed to efficiently protect antibodies against perturbation environments and quickly recover them from the MOFs before usage, which avoids introducing protective additives into the body, which may cause biosafety risks. The protected antibodies exhibit extraordinary thermal, chemical, and mechanical stabilities, and they can survive for long-term storage (>3 weeks) under severe temperature variation (4 ↔ 50 °C) at a fast ramp rate (25 °C min −1 ). More importantly, the encapsulated antibodies can be easily released as quickly as 10 s with high efficiency (≈100%) to completely remove the MOFs before use. This study paves a new avenue for the facile preparation and storage of biopharmaceuticals represented by antibodies under ambient or perturbation conditions, which may greatly broaden and promote the applications of both MOFs and biopharmaceuticals.
AB - Antibodies have emerged as a fast-growing category of biopharmaceuticals that have been widely applied in scientific research, medical diagnosis, and disease treatment. However, many antibodies and other biopharmaceuticals display inferior biophysical properties, such as low stability and a propensity to undergo aggregation. Enhancing the stability of biopharmaceuticals is essential for their wide applications. Here, a facile in vitro protective coating strategy based on metal–organic frameworks (MOFs) is proposed to efficiently protect antibodies against perturbation environments and quickly recover them from the MOFs before usage, which avoids introducing protective additives into the body, which may cause biosafety risks. The protected antibodies exhibit extraordinary thermal, chemical, and mechanical stabilities, and they can survive for long-term storage (>3 weeks) under severe temperature variation (4 ↔ 50 °C) at a fast ramp rate (25 °C min −1 ). More importantly, the encapsulated antibodies can be easily released as quickly as 10 s with high efficiency (≈100%) to completely remove the MOFs before use. This study paves a new avenue for the facile preparation and storage of biopharmaceuticals represented by antibodies under ambient or perturbation conditions, which may greatly broaden and promote the applications of both MOFs and biopharmaceuticals.
KW - antibodies
KW - biopharmaceuticals
KW - encapsulate
KW - metal–organic frameworks
KW - release
UR - http://www.scopus.com/inward/record.url?scp=85057484839&partnerID=8YFLogxK
U2 - 10.1002/adma.201805148
DO - 10.1002/adma.201805148
M3 - Article
C2 - 30480344
AN - SCOPUS:85057484839
SN - 0935-9648
VL - 31
SP - e1805148
JO - Advanced Materials
JF - Advanced Materials
IS - 2
M1 - 1805148
ER -