Abstract
A Ficus carica L. latex proteinase preparation was investigated for its ability to produce antioxidant hydrolysates/peptides from bovine casein (CN). The Oxygen Radical Absorbance Capacity (ORAC) values for NaCN and β-CN hydrolysates ranged from 0.06 to 0.18, and from 0.51 to 1.19 μmol Trolox equivalents/mg freeze-dried sample, respectively. Gel permeation HPLC showed that the β-CN hydrolysate with a degree of hydrolysis of 21% had 65% of peptide material with a molecular mass <500 Da. The RP-UPLC profiles also indicated that β-CN was substantially hydrolysed during the early stages of hydrolysis. Analysis of the 4 h β-CN hydrolysate by LC-ESI-MS/MS allowed identification of 8 peptide sequences with potential antioxidant properties.
Original language | English |
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Pages (from-to) | 305-311 |
Number of pages | 7 |
Journal | Food Chemistry |
Volume | 156 |
DOIs | |
Publication status | Published - 1 Aug 2014 |
Keywords
- β-Casein
- Antioxidant activity
- Bioactive peptides
- Degree of hydrolysis
- Ficus carica L.
- Mass spectrometry
- ORAC assay
- Sodium caseinate