TY - JOUR
T1 - Antioxidant activity, total phenolics and flavonoids contents
T2 - Should we ban in vitro screening methods?
AU - Granato, Daniel
AU - Shahidi, Fereidoon
AU - Wrolstad, Ronald
AU - Kilmartin, Paul
AU - Melton, Laurence D.
AU - Hidalgo, Francisco J.
AU - Miyashita, Kazuo
AU - Camp, John van
AU - Alasalvar, Cesarettin
AU - Ismail, Amin B.
AU - Elmore, Stephen
AU - Birch, Gordon G.
AU - Charalampopoulos, Dimitris
AU - Astley, Sian B.
AU - Pegg, Ronald
AU - Zhou, Peng
AU - Finglas, Paul
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/10/30
Y1 - 2018/10/30
N2 - As many studies are exploring the association between ingestion of bioactive compounds and decreased risk of non-communicable diseases, the scientific community continues to show considerable interest in these compounds. In addition, as many non-nutrients with putative health benefits are reducing agents, hydrogen donors, singlet oxygen quenchers or metal chelators, measurement of antioxidant activity using in vitro assays has become very popular over recent decades. Measuring concentrations of total phenolics, flavonoids, and other compound (sub)classes using UV/Vis spectrophotometry offers a rapid chemical index, but chromatographic techniques are necessary to establish structure-activity. For bioactive purposes, in vivo models are required or, at the very least, methods that employ distinct mechanisms of action (i.e., single electron transfer, transition metal chelating ability, and hydrogen atom transfer). In this regard, better understanding and application of in vitro screening methods should help design of future research studies on ‘bioactive compounds’.
AB - As many studies are exploring the association between ingestion of bioactive compounds and decreased risk of non-communicable diseases, the scientific community continues to show considerable interest in these compounds. In addition, as many non-nutrients with putative health benefits are reducing agents, hydrogen donors, singlet oxygen quenchers or metal chelators, measurement of antioxidant activity using in vitro assays has become very popular over recent decades. Measuring concentrations of total phenolics, flavonoids, and other compound (sub)classes using UV/Vis spectrophotometry offers a rapid chemical index, but chromatographic techniques are necessary to establish structure-activity. For bioactive purposes, in vivo models are required or, at the very least, methods that employ distinct mechanisms of action (i.e., single electron transfer, transition metal chelating ability, and hydrogen atom transfer). In this regard, better understanding and application of in vitro screening methods should help design of future research studies on ‘bioactive compounds’.
KW - Antioxidants
KW - Bioavailability
KW - Colorimetric methods
KW - Folin-Ciocalteu
KW - Functional properties
KW - HPLC
KW - In vivo studies
UR - http://www.scopus.com/inward/record.url?scp=85045564608&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2018.04.012
DO - 10.1016/j.foodchem.2018.04.012
M3 - Article
C2 - 29853403
AN - SCOPUS:85045564608
SN - 0308-8146
VL - 264
SP - 471
EP - 475
JO - Food Chemistry
JF - Food Chemistry
ER -