TY - JOUR
T1 - Antioxidant effects of enzymatic hydrolysates of whey protein concentrate on cultured human endothelial cells
AU - O'Keeffe, Martina B.
AU - FitzGerald, Richard J.
PY - 2014/6
Y1 - 2014/6
N2 - Enzymatic hydrolysis of whey protein concentrate (WPC) was performed using Alcalase, Neutrase, Corolase PP or Flavourzyme. The hydrolysates were filtered through membranes having different molecular mass cut-offs. The hydrolysate fractions had significantly (P<0.001) greater antioxidant capacity than unhydrolysed WPC, as measured by the oxygen radical absorbance capacity assay (ORAC). Furthermore, incubation of human umbilical vein endothelial cells (HUVECs) with the hydrolysate fractions resulted in an increase in cellular glutathione and catalase activity. Microarray analysis showed beneficial regulation of various genes involved in the antioxidant/detoxification processes in HUVECs incubated with the 5kDa permeates of WPC hydrolysed by Alcalase or Neutrase. In conclusion, peptides generated by the enzymatic hydrolysis of WPC using Alcalase, Neutrase, Corolase PP or Flavourzyme have a high antioxidant capacity and may have a role in the beneficial regulation of endothelial cell function.
AB - Enzymatic hydrolysis of whey protein concentrate (WPC) was performed using Alcalase, Neutrase, Corolase PP or Flavourzyme. The hydrolysates were filtered through membranes having different molecular mass cut-offs. The hydrolysate fractions had significantly (P<0.001) greater antioxidant capacity than unhydrolysed WPC, as measured by the oxygen radical absorbance capacity assay (ORAC). Furthermore, incubation of human umbilical vein endothelial cells (HUVECs) with the hydrolysate fractions resulted in an increase in cellular glutathione and catalase activity. Microarray analysis showed beneficial regulation of various genes involved in the antioxidant/detoxification processes in HUVECs incubated with the 5kDa permeates of WPC hydrolysed by Alcalase or Neutrase. In conclusion, peptides generated by the enzymatic hydrolysis of WPC using Alcalase, Neutrase, Corolase PP or Flavourzyme have a high antioxidant capacity and may have a role in the beneficial regulation of endothelial cell function.
UR - http://www.scopus.com/inward/record.url?scp=84896878189&partnerID=8YFLogxK
U2 - 10.1016/j.idairyj.2014.01.013
DO - 10.1016/j.idairyj.2014.01.013
M3 - Article
AN - SCOPUS:84896878189
SN - 0958-6946
VL - 36
SP - 128
EP - 135
JO - International Dairy Journal
JF - International Dairy Journal
IS - 2
ER -