TY - JOUR
T1 - Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry
AU - Burdikova, Zuzana
AU - Svindrych, Zdenek
AU - Hickey, Cian
AU - Wilkinson, Martin G.
AU - Auty, Mark A.E.
AU - Samek, Ota
AU - Bernatova, Silvia
AU - Krzyzanek, Vladislav
AU - Periasamy, Ammasi
AU - Sheehan, Jeremiah J.
N1 - Publisher Copyright:
© 2015, INRA and Springer-Verlag France.
PY - 2015/9/23
Y1 - 2015/9/23
N2 - Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.
AB - Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.
KW - Cheese matrix
KW - Confocal microscopy
KW - Confocal Raman microscopy
KW - Fluorescence lifetime
KW - Second harmonic generation
KW - Two-photon excitation
UR - http://www.scopus.com/inward/record.url?scp=84941954877&partnerID=8YFLogxK
U2 - 10.1007/s13594-015-0253-2
DO - 10.1007/s13594-015-0253-2
M3 - Article
AN - SCOPUS:84941954877
SN - 1958-5586
VL - 95
SP - 687
EP - 700
JO - Dairy Science and Technology
JF - Dairy Science and Technology
IS - 5
ER -
