Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Zuzana Burdikova; Zdenek Svindrych; Cian Hickey; Martin G. Wilkinson; Mark A.E. Auty; Ota Samek; Silvia Bernatova; Vladislav Krzyzanek; Ammasi Periasamy; Jeremiah J. Sheehan

doi:10.1007/s13594-015-0253-2

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Zuzana Burdikova
, Zdenek Svindrych
, Cian Hickey
, Martin G. Wilkinson
, Mark A.E. Auty
, Ota Samek
, Silvia Bernatova
, Vladislav Krzyzanek
, Ammasi Periasamy
, Jeremiah J. Sheehan

Department of Biological Sciences

Teagasc - Irish Agriculture and Food Development Authority
University of Virginia
Brno University of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

Original language	English
Pages (from-to)	687-700
Number of pages	14
Journal	Dairy Science and Technology
Volume	95
Issue number	5
DOIs	https://doi.org/10.1007/s13594-015-0253-2
Publication status	Published - 23 Sep 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Keywords

Cheese matrix
Confocal Raman microscopy
Confocal microscopy
Fluorescence lifetime
Second harmonic generation
Two-photon excitation

Access to Document

10.1007/s13594-015-0253-2

Cite this

@article{4ff430d7ee25497f8c9e3af8f39bba9f,

title = "Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry",

abstract = "Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.",

keywords = "Cheese matrix, Confocal Raman microscopy, Confocal microscopy, Fluorescence lifetime, Second harmonic generation, Two-photon excitation",

author = "Zuzana Burdikova and Zdenek Svindrych and Cian Hickey and Wilkinson, \{Martin G.\} and Auty, \{Mark A.E.\} and Ota Samek and Silvia Bernatova and Vladislav Krzyzanek and Ammasi Periasamy and Sheehan, \{Jeremiah J.\}",

note = "Publisher Copyright: {\textcopyright} 2015, INRA and Springer-Verlag France.",

year = "2015",

month = sep,

day = "23",

doi = "10.1007/s13594-015-0253-2",

language = "English",

volume = "95",

pages = "687--700",

journal = "Dairy Science and Technology",

issn = "1958-5586",

number = "5",

}

TY - JOUR

T1 - Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

AU - Burdikova, Zuzana

AU - Svindrych, Zdenek

AU - Hickey, Cian

AU - Wilkinson, Martin G.

AU - Auty, Mark A.E.

AU - Samek, Ota

AU - Bernatova, Silvia

AU - Krzyzanek, Vladislav

AU - Periasamy, Ammasi

AU - Sheehan, Jeremiah J.

PY - 2015/9/23

Y1 - 2015/9/23

N2 - Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

AB - Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

KW - Cheese matrix

KW - Confocal Raman microscopy

KW - Confocal microscopy

KW - Fluorescence lifetime

KW - Second harmonic generation

KW - Two-photon excitation

UR - https://www.scopus.com/pages/publications/84941954877

U2 - 10.1007/s13594-015-0253-2

DO - 10.1007/s13594-015-0253-2

M3 - Article

AN - SCOPUS:84941954877

SN - 1958-5586

VL - 95

SP - 687

EP - 700

JO - Dairy Science and Technology

JF - Dairy Science and Technology

IS - 5

ER -

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this