Application of Human Plasma Targeted Lipidomics and Analysis of Toxic Elements to Capture the Metabolic Complexities of Hypothyroidism

Anna Błażewicz , Michał Kiełbus , Katarzyna Skórzyńska-Dziduszko, Andreas Grabrucker, Jacqueline Jonklaas, Piotr Sosnowski , Alicja Trzpil, Anna Kozub-Pędrak, Agnieszka Szmagara, Julia Wojnicka , Ewelina Grywalska, Agostinho Almeida

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Hypothyroidism (HT) affects millions worldwide and can lead to various lipid disorders. The metabolic complexity and the influence of toxic elements in autoimmune and non-auto- immune HT subtypes are not fully understood. This study aimed to investigate the relationships between plasma lipidome, toxic elements, and clinical classifications of HT in unexposed individuals. Methods: Samples were collected from 120 adults assigned to a study group with Hashimoto’s disease and non- autoimmune HT, and a healthy control group. Quantification of 145 pre-defined lipids was performed by using triple quadrupole tandem mass spectrometry (TQ MS/MS) in multiple reactions monitoring (MRM) mode via positive electrospray ionization (ESI). Levels of toxic elements were determined using inductively coupled plasma mass spectrometry (ICP-MS). Results: Significant associations between al- tered levels of several components of the plasma lipidome and Al, Cd, Ni, As, and Pb with HT were found. We show metabolic differences in lysophosphatidylcholines (LPC) and phosphatidylcholines (PC) between HT and controls, with distinct predicted activation patterns for lysolecithin acyltransferase and phospholipase A2. Conclusions: There are significant changes in the lipidome profiles of healthy subjects compared to euthyroid HT patients treated with L-thyroxine, which are related to the type of hypothyroidism and non-occupational exposure to toxic elements.
Original languageEnglish (Ireland)
Article number5169
JournalMolecules
Volume29
Issue number21
DOIs
Publication statusPublished - 31 Oct 2024

Keywords

  • human plasma lipidome
  • Hashimoto disease
  • hypothyroidism
  • toxic elements
  • metals

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