TY - JOUR
T1 - Arbuscular mycorrhizal hyphae in grassland select for a diverse and abundant hyphospheric bacterial community involved in sulfonate desulfurization
AU - Gahan, Jacinta
AU - Schmalenberger, Achim
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Hyphae of symbiotic arbuscular mycorrhizal (AM) fungi extend into the soil, affecting the hyphosphere and interact with beneficial soil bacteria. This study aimed to elucidate differences in hyphosphere, hyphoplane and bulk soil bacterial communities and their role in mobilization of sulfonate-sulfur. Abundances of cultivable hyphosphere and hyphoplane bacteria were significantly increased over bulk soil. Cultivation independent fingerprinting revealed significantly different community structures of both hyphosphere and hyphoplane bacteria, fungi and AM fungi over bulk soil. However, cultivation dependent and independent analysis did not identify a difference between bacterial hyphoplane and hyphosphere (hyphospheric) communities. Isolated bacteria capable of aromatic sulfonate desulfurization were almost exclusively of hyphospheric origin. Members of the hyphospheric Gammaproteobacteria and Actinobacteria were found to possess marker gene asfA for aromatic sulfonate desulfurization and hrcR for attachment to fungal hyphae with a type III secretion system, that were not detected in bulk soil. These findings suggest that AM hyphae host a distinct population of sulfonate desulfurizing bacteria putatively capable of hyphal attachment with potential to increase plant sulfur supply.
AB - Hyphae of symbiotic arbuscular mycorrhizal (AM) fungi extend into the soil, affecting the hyphosphere and interact with beneficial soil bacteria. This study aimed to elucidate differences in hyphosphere, hyphoplane and bulk soil bacterial communities and their role in mobilization of sulfonate-sulfur. Abundances of cultivable hyphosphere and hyphoplane bacteria were significantly increased over bulk soil. Cultivation independent fingerprinting revealed significantly different community structures of both hyphosphere and hyphoplane bacteria, fungi and AM fungi over bulk soil. However, cultivation dependent and independent analysis did not identify a difference between bacterial hyphoplane and hyphosphere (hyphospheric) communities. Isolated bacteria capable of aromatic sulfonate desulfurization were almost exclusively of hyphospheric origin. Members of the hyphospheric Gammaproteobacteria and Actinobacteria were found to possess marker gene asfA for aromatic sulfonate desulfurization and hrcR for attachment to fungal hyphae with a type III secretion system, that were not detected in bulk soil. These findings suggest that AM hyphae host a distinct population of sulfonate desulfurizing bacteria putatively capable of hyphal attachment with potential to increase plant sulfur supply.
KW - Arbuscular mycorrhiza
KW - Bacterial community
KW - Hyphosphere
KW - Sulfur mobilization
UR - http://www.scopus.com/inward/record.url?scp=84921867582&partnerID=8YFLogxK
U2 - 10.1016/j.apsoil.2014.12.008
DO - 10.1016/j.apsoil.2014.12.008
M3 - Article
AN - SCOPUS:84921867582
SN - 0929-1393
VL - 89
SP - 113
EP - 121
JO - Applied Soil Ecology
JF - Applied Soil Ecology
ER -