TY - JOUR
T1 - Assaying polymorphism by intron targeted intron-exon specific junction (ISJ) DNA marker for genetic diversity diagnostics of the Cucumis L. taxa
AU - Rajkumari, Khedasana
AU - Sharma, Santosh Kumar
AU - Rao, Satyawada Rama
PY - 2013/4
Y1 - 2013/4
N2 - Forty five genotypes of cultivated, wild, newly described Cucumis L. species with special emphasis on Indian representatives and the taxa transferred from other genera to Cucumis were analyzed for analysis of genetic variation using Intron-exon Spliced Junction (ISJ) marker at inter- and intra- specific levels. A total of 152 amplification products were scored by 10 ISJ primers with 15.2 amplicons per primer which collectively revealed 99.34 % polymorphism at inter-specific level. Cucumis species, C. sativus, C. sativus var. hardwickii, C. callosus, and C. melo along with 4 varieties of C. melo viz. melo, conomon, momordica and agrestis were also analyzed to assess the genetic variation at intra-specific level. The average percentage of polymorphic loci ranged between 88.13 (C. sativus) and 92.20 (C. sativus var. hardwickii). Ten ISJ primers collectively revealed 9 unique species-specific amplicons. Most of them showed specificity towards wild species. Two unique and conspicuous bands proved explicit towards C. silentvalleyii and C. hystrix. The UPGMA phenogram generated through cumulative dataset of 10 ISJ primers using Jaccard's coefficients of similarity distinguished the taxa into three major clusters. The grouping was generally congruent with zygotic chromosome numbers reported in the genus. The present investigation provides a valuable baseline data for existing genetic variation among Indian representative wild and cultivated species/ varieties of Cucumis and addresses concern to its sustainable utilization and further improvement.
AB - Forty five genotypes of cultivated, wild, newly described Cucumis L. species with special emphasis on Indian representatives and the taxa transferred from other genera to Cucumis were analyzed for analysis of genetic variation using Intron-exon Spliced Junction (ISJ) marker at inter- and intra- specific levels. A total of 152 amplification products were scored by 10 ISJ primers with 15.2 amplicons per primer which collectively revealed 99.34 % polymorphism at inter-specific level. Cucumis species, C. sativus, C. sativus var. hardwickii, C. callosus, and C. melo along with 4 varieties of C. melo viz. melo, conomon, momordica and agrestis were also analyzed to assess the genetic variation at intra-specific level. The average percentage of polymorphic loci ranged between 88.13 (C. sativus) and 92.20 (C. sativus var. hardwickii). Ten ISJ primers collectively revealed 9 unique species-specific amplicons. Most of them showed specificity towards wild species. Two unique and conspicuous bands proved explicit towards C. silentvalleyii and C. hystrix. The UPGMA phenogram generated through cumulative dataset of 10 ISJ primers using Jaccard's coefficients of similarity distinguished the taxa into three major clusters. The grouping was generally congruent with zygotic chromosome numbers reported in the genus. The present investigation provides a valuable baseline data for existing genetic variation among Indian representative wild and cultivated species/ varieties of Cucumis and addresses concern to its sustainable utilization and further improvement.
KW - Cucumis
KW - ISJ Polymorphism
KW - Species-specific amplicon
KW - Wild species
UR - http://www.scopus.com/inward/record.url?scp=84880611968&partnerID=8YFLogxK
U2 - 10.1007/s13237-013-0080-x
DO - 10.1007/s13237-013-0080-x
M3 - Article
AN - SCOPUS:84880611968
SN - 0029-568X
VL - 56
SP - 15
EP - 21
JO - Nucleus (India)
JF - Nucleus (India)
IS - 1
ER -