Assaying polymorphism by intron targeted intron-exon specific junction (ISJ) DNA marker for genetic diversity diagnostics of the Cucumis L. taxa

Khedasana Rajkumari, Santosh Kumar Sharma, Satyawada Rama Rao

Research output: Contribution to journalArticlepeer-review

Abstract

Forty five genotypes of cultivated, wild, newly described Cucumis L. species with special emphasis on Indian representatives and the taxa transferred from other genera to Cucumis were analyzed for analysis of genetic variation using Intron-exon Spliced Junction (ISJ) marker at inter- and intra- specific levels. A total of 152 amplification products were scored by 10 ISJ primers with 15.2 amplicons per primer which collectively revealed 99.34 % polymorphism at inter-specific level. Cucumis species, C. sativus, C. sativus var. hardwickii, C. callosus, and C. melo along with 4 varieties of C. melo viz. melo, conomon, momordica and agrestis were also analyzed to assess the genetic variation at intra-specific level. The average percentage of polymorphic loci ranged between 88.13 (C. sativus) and 92.20 (C. sativus var. hardwickii). Ten ISJ primers collectively revealed 9 unique species-specific amplicons. Most of them showed specificity towards wild species. Two unique and conspicuous bands proved explicit towards C. silentvalleyii and C. hystrix. The UPGMA phenogram generated through cumulative dataset of 10 ISJ primers using Jaccard's coefficients of similarity distinguished the taxa into three major clusters. The grouping was generally congruent with zygotic chromosome numbers reported in the genus. The present investigation provides a valuable baseline data for existing genetic variation among Indian representative wild and cultivated species/ varieties of Cucumis and addresses concern to its sustainable utilization and further improvement.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalNucleus (India)
Volume56
Issue number1
DOIs
Publication statusPublished - Apr 2013
Externally publishedYes

Keywords

  • Cucumis
  • ISJ Polymorphism
  • Species-specific amplicon
  • Wild species

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