Abstract
Objective: To determine the effect on nitric oxide (NO release and renal NO synthase (endothelial, eNOS and inducible, iNOS) activity of renal ischaemia-reperfusion (I/R) in vivo in an animal model, and to examine the possible involvement of NO in ischaemic preconditioning (IP) of the kidney. Materials and methods: In a right-nephrectomized rat model, 42 animals were randomized in four groups: controls; IP-only (4 min of ischaemia followed by 11 min of reperfusion, total of four cycles): renal warm ischaemia (45 min) and 6 h reperfusion: ischaemia (45 min) preceded by IT pretreatment. Serum NO metabolites were assayed 2 and 6 h after ischaemia or the control equivalent. NOS expression in the kidney was detected immuno-histochemically, and damage assessed morphologically in sections stained with haematoxylin and eosin. Kidney function was assessed by the levels of serum creatinine, urea and electrolytes. Results: Compared with before ischaemia, the concentration of serum NO metabolites at 6 h was increased in the IP-only animals (P = 0.016) and in the IP + I/R group (P = 0.002). There was greater eNOS expression in the IP-only group (P = 0.009) and in the IP + I/R group than in controls (P = 0.050). iNOS expression was greater in the IP-only animals than in the control group (P = 0.050)). Histological assessment showed less evidence of cellular damage in IP + I/R animals than in the IR-alone group (P = 0.020), Serum creatinine level was not significantly different between the IP-only group and the control. There were no differences after 2 h of reperfusion. Conclusion: Ischaemic preconditioning has a protective effect on renal structure and function, which may be produced by increased NO release arising from increased NOS expression by 6 h after reperfusion.
Original language | English |
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Pages (from-to) | 1007-1013 |
Number of pages | 7 |
Journal | BJU International |
Volume | 85 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2000 |
Externally published | Yes |
Keywords
- Ischaemia
- Kidney
- Nitric oxide
- Preconditioning
- Reperfusion