Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform

David J. Kinahan; Faith Mangwanya; Robert Garvey; Danielle W.Y. Chung; Artur Lipinski; Lourdes A.N. Julius; Damien King; Mehdi Mohammadi; Rohit Mishra; May Al-Ofi; Celina Miyazaki; Jens Ducrée

doi:10.1088/1742-6596/757/1/012013

Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform

David J. Kinahan, Faith Mangwanya, Robert Garvey, Danielle W.Y. Chung, Artur Lipinski, Lourdes A.N. Julius, Damien King, Mehdi Mohammadi, Rohit Mishra, May Al-Ofi, Celina Miyazaki, Jens Ducrée

Dublin City University

Research output: Contribution to journal › Conference article › peer-review

Abstract

We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.

Original language	English
Article number	012013
Journal	Journal of Physics: Conference Series
Volume	757
Issue number	1
DOIs	https://doi.org/10.1088/1742-6596/757/1/012013
Publication status	Published - 11 Nov 2016
Externally published	Yes
Event	27th Micromechanics and Microsystems Europe Workshop, MME 2016 - Cork, Ireland Duration: 28 Aug 2016 → 30 Aug 2016

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Kinahan, D. J., Mangwanya, F., Garvey, R., Chung, D. W. Y., Lipinski, A., Julius, L. A. N., King, D., Mohammadi, M., Mishra, R., Al-Ofi, M., Miyazaki, C., & Ducrée, J. (2016). Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform. Journal of Physics: Conference Series, 757(1), Article 012013. https://doi.org/10.1088/1742-6596/757/1/012013

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title = "Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform",

abstract = "We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.",

author = "Kinahan, {David J.} and Faith Mangwanya and Robert Garvey and Chung, {Danielle W.Y.} and Artur Lipinski and Julius, {Lourdes A.N.} and Damien King and Mehdi Mohammadi and Rohit Mishra and May Al-Ofi and Celina Miyazaki and Jens Ducr{\'e}e",

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doi = "10.1088/1742-6596/757/1/012013",

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AU - Kinahan, David J.

AU - Mangwanya, Faith

AU - Garvey, Robert

AU - Chung, Danielle W.Y.

AU - Lipinski, Artur

AU - Julius, Lourdes A.N.

AU - King, Damien

AU - Mohammadi, Mehdi

AU - Mishra, Rohit

AU - Al-Ofi, May

AU - Miyazaki, Celina

AU - Ducrée, Jens

PY - 2016/11/11

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N2 - We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.

AB - We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.

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DO - 10.1088/1742-6596/757/1/012013

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SN - 1742-6588

VL - 757

JO - Journal of Physics: Conference Series

JF - Journal of Physics: Conference Series

IS - 1

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T2 - 27th Micromechanics and Microsystems Europe Workshop, MME 2016

Y2 - 28 August 2016 through 30 August 2016

ER -

Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform

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