Azeotropic phenomena during separation of dilute mixtures of proteins by simulated moving bed chromatography

Joukje Houwing; Hugo A.H. Billiet; Hans A. Wesselingh; Luuk A.M. Van Der Wielen

doi:10.1002/(SICI)1097-4660(199903)74:3<213::AID-JCTB46>3.0.CO;2-M

Azeotropic phenomena during separation of dilute mixtures of proteins by simulated moving bed chromatography

Joukje Houwing, Hugo A.H. Billiet, Hans A. Wesselingh, Luuk A.M. Van Der Wielen

Delft University of Technology

Research output: Contribution to journal › Conference article › peer-review

Abstract

Recently, we have introduced the use of a salt gradient over a simulated moving bed system used for purification of proteins using ion exchange chromatography. This paper describes azeotropic phenomena in such a system, where complete separation of mixtures of proteins is not possible at certain salt concentrations, due to a reversing selectivity. These phenomena impose additional restrictions on the selection of the flow rates in each of the sections of the simulated moving bed which are discussed.

Original language	English
Pages (from-to)	213-215
Number of pages	3
Journal	Journal of Chemical Technology and Biotechnology
Volume	74
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1097-4660(199903)74:3<213::AID-JCTB46>3.0.CO;2-M
Publication status	Published - Mar 1999
Externally published	Yes
Event	Proceedings of the 1999 4th International Conference on Separations for Biotechnology - Reading, GBR Duration: 29 Mar 1999 → 31 Mar 1999

Keywords

Azeotrope
Bioseparation
Moving bed chromatography

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10.1002/(SICI)1097-4660(199903)74:3<213::AID-JCTB46>3.0.CO;2-M

Cite this

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title = "Azeotropic phenomena during separation of dilute mixtures of proteins by simulated moving bed chromatography",

abstract = "Recently, we have introduced the use of a salt gradient over a simulated moving bed system used for purification of proteins using ion exchange chromatography. This paper describes azeotropic phenomena in such a system, where complete separation of mixtures of proteins is not possible at certain salt concentrations, due to a reversing selectivity. These phenomena impose additional restrictions on the selection of the flow rates in each of the sections of the simulated moving bed which are discussed.",

keywords = "Azeotrope, Bioseparation, Moving bed chromatography",

author = "Joukje Houwing and Billiet, \{Hugo A.H.\} and Wesselingh, \{Hans A.\} and \{Van Der Wielen\}, \{Luuk A.M.\}",

year = "1999",

month = mar,

doi = "10.1002/(SICI)1097-4660(199903)74:3<213::AID-JCTB46>3.0.CO;2-M",

language = "English",

volume = "74",

pages = "213--215",

journal = "Journal of Chemical Technology and Biotechnology",

issn = "0268-2575",

number = "3",

note = "Proceedings of the 1999 4th International Conference on Separations for Biotechnology ; Conference date: 29-03-1999 Through 31-03-1999",

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TY - JOUR

T1 - Azeotropic phenomena during separation of dilute mixtures of proteins by simulated moving bed chromatography

AU - Houwing, Joukje

AU - Billiet, Hugo A.H.

AU - Wesselingh, Hans A.

AU - Van Der Wielen, Luuk A.M.

PY - 1999/3

Y1 - 1999/3

N2 - Recently, we have introduced the use of a salt gradient over a simulated moving bed system used for purification of proteins using ion exchange chromatography. This paper describes azeotropic phenomena in such a system, where complete separation of mixtures of proteins is not possible at certain salt concentrations, due to a reversing selectivity. These phenomena impose additional restrictions on the selection of the flow rates in each of the sections of the simulated moving bed which are discussed.

AB - Recently, we have introduced the use of a salt gradient over a simulated moving bed system used for purification of proteins using ion exchange chromatography. This paper describes azeotropic phenomena in such a system, where complete separation of mixtures of proteins is not possible at certain salt concentrations, due to a reversing selectivity. These phenomena impose additional restrictions on the selection of the flow rates in each of the sections of the simulated moving bed which are discussed.

KW - Azeotrope

KW - Bioseparation

KW - Moving bed chromatography

UR - https://www.scopus.com/pages/publications/0032982429

U2 - 10.1002/(SICI)1097-4660(199903)74:3<213::AID-JCTB46>3.0.CO;2-M

DO - 10.1002/(SICI)1097-4660(199903)74:3<213::AID-JCTB46>3.0.CO;2-M

M3 - Conference article

AN - SCOPUS:0032982429

SN - 0268-2575

VL - 74

SP - 213

EP - 215

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

IS - 3

T2 - Proceedings of the 1999 4th International Conference on Separations for Biotechnology

Y2 - 29 March 1999 through 31 March 1999

ER -

Azeotropic phenomena during separation of dilute mixtures of proteins by simulated moving bed chromatography

Abstract

Keywords

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