TY - JOUR
T1 - Bioelectrochemistry of Cytochrome c in a closed bipolar electrochemical cell
AU - Gamero-Quijano, Alonso
AU - Herzog, Grégoire
AU - Scanlon, Micheál D.
N1 - Publisher Copyright:
© 2019 The Authors
PY - 2019/12
Y1 - 2019/12
N2 - The reversible oxidation and reduction of Cytochrome c (Cyt c) is demonstrated with a closed bipolar electrochemical cell (CBPEC). Herein, a 4-electrode configuration was studied with the opposite poles of the bipolar electrode resting in separate aqueous and organic electrolyte solutions, respectively. Using biocompatible indium tin oxide (ITO) slides as the bipolar electrode poles, we investigated the influence of the redox potential of the reductant (decamethyferrocene or dimethylferrocene) in an α,α,α-trifluorotoluene organic phase on the observed voltammetry. Reversible electron transfer was only observed between Cyt c and decamethylferrocene. Use of the weaker dimethylferrocene as the reductant required a larger external bias of the driving electrodes to initiate the electron transfer reaction between the two poles of the bipolar electrode. Consequently, the surface of the ITO slide at the aqueous pole experienced a significant negative cathodic potential and underwent irreversible reduction. The biphasic setup using the 4-electrode CBPEC provides insights into electron transfer processes at an interface between two immiscible electrolyte solutions (ITIES), highlighting the strong probability of observing interfacial electron transfer between decamethylferrocene (but not dimethylferrocene) and Cyt c within the short ~1 V polarisable potential window available at an ITIES.
AB - The reversible oxidation and reduction of Cytochrome c (Cyt c) is demonstrated with a closed bipolar electrochemical cell (CBPEC). Herein, a 4-electrode configuration was studied with the opposite poles of the bipolar electrode resting in separate aqueous and organic electrolyte solutions, respectively. Using biocompatible indium tin oxide (ITO) slides as the bipolar electrode poles, we investigated the influence of the redox potential of the reductant (decamethyferrocene or dimethylferrocene) in an α,α,α-trifluorotoluene organic phase on the observed voltammetry. Reversible electron transfer was only observed between Cyt c and decamethylferrocene. Use of the weaker dimethylferrocene as the reductant required a larger external bias of the driving electrodes to initiate the electron transfer reaction between the two poles of the bipolar electrode. Consequently, the surface of the ITO slide at the aqueous pole experienced a significant negative cathodic potential and underwent irreversible reduction. The biphasic setup using the 4-electrode CBPEC provides insights into electron transfer processes at an interface between two immiscible electrolyte solutions (ITIES), highlighting the strong probability of observing interfacial electron transfer between decamethylferrocene (but not dimethylferrocene) and Cyt c within the short ~1 V polarisable potential window available at an ITIES.
KW - Bioelectrochemistry
KW - Bipolar electrochemistry
KW - Bipolar electrode
KW - Cytochrome c
KW - Four electrode configuration
UR - http://www.scopus.com/inward/record.url?scp=85074632973&partnerID=8YFLogxK
U2 - 10.1016/j.elecom.2019.106600
DO - 10.1016/j.elecom.2019.106600
M3 - Article
AN - SCOPUS:85074632973
SN - 1388-2481
VL - 109
JO - Electrochemistry Communications
JF - Electrochemistry Communications
M1 - 106600
ER -