Bioseparations in aqueous micellar systems based on excluded-volume interactions

D. Van Roosmalen, M. P.J. Dohmen-Speelmans, C. H.J.T. Dietz, L. J.P. van den Broeke, L. A.M. van der Wielen, J. T.F. Keurentjes

Research output: Contribution to journalArticlepeer-review

Abstract

Protein partitioning based on micellar-induced excluded-volume interactions is studied for different aqueous nonionic micellar systems. In particular, protein partitioning in the traditional aqueous micellar two-phase system is compared with a system where a gel phase is combined with a micellar phase and with a membrane system with micelles at the feed side of the membrane. A two-phase system is created using cylindrically-shaped n-decyl tetra(ethylene oxide) (C10E4) micelles that are too large to diffuse into the gel-bead or across the membrane. Results are reported for the partition coefficient of single-component protein systems with myoglobin, ovalbumin, and BSA, and for binary protein mixtures. The individual protein partition coefficients in the mixtures follow the same protein concentration dependence as the partition coefficient obtained for the single-component protein solutions.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalFood and Bioproducts Processing
Volume84
Issue number1 C
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Keywords

  • Excluded-volume interactions
  • Membrane
  • Nonionic surfactant
  • Partitioning
  • Protein
  • Sephacryl gel

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