TY - JOUR
T1 - Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay
AU - Baez-Jurado, Eliana
AU - Vega, Gina Guio
AU - Aliev, Gjumrakch
AU - Tarasov, Vadim V.
AU - Esquinas, Paula
AU - Echeverria, Valentina
AU - Barreto, George E.
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media New York.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies.
AB - Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies.
KW - Adipose tissue
KW - Astrocytes
KW - Conditioned medium
KW - Mesenchymal stem cells
KW - Neuroglobin
KW - Scratch assay
UR - http://www.scopus.com/inward/record.url?scp=85015892254&partnerID=8YFLogxK
U2 - 10.1007/s12035-017-0481-y
DO - 10.1007/s12035-017-0481-y
M3 - Article
C2 - 28332151
AN - SCOPUS:85015892254
SN - 0893-7648
VL - 55
SP - 2285
EP - 2300
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 3
ER -