TY - JOUR
T1 - Camu-camu (Myrciaria dubia) seeds as a novel source of bioactive compounds with promising antimalarial and antischistosomicidal properties
AU - do Carmo, Mariana Araújo Vieira
AU - Fidelis, Marina
AU - Sanchez, Caroline Andolfato
AU - Castro, Aline Pereira
AU - Camps, Ihosvany
AU - Colombo, Fábio Antônio
AU - Marques, Marcos José
AU - Myoda, Takao
AU - Granato, Daniel
AU - Azevedo, Luciana
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/10
Y1 - 2020/10
N2 - Parasitic diseases have attracted worldwide attention of their consequent impact on mortality and morbidity. Accordingly, several plants have been screened for antiparasitic activity aiming to create new alternatives for treatment. These diseases have been neglected and have not attracted worldwide attention (nowadays), the health concerns are focused in chronic diseases, but it is necessary to focus on parasitic diseases and look for prophylactic alternatives, such as plant extracts. Although camu-camu (Myrciaria dubia) seeds are a rich source of antioxidant antimutagenic, cytotoxic, anti-inflammatory, antimicrobial, antihypertensive and neuroprotective compounds, nothing is known about their antiparasitic effects. Thus, in the present study we aimed to evaluate five extracts of camu-camu seeds (100% water, 100% ethyl alcohol, 50% water + 50% ethyl alcohol, 25% water + 75% ethyl alcohol, and 75% water + 25% ethyl alcohol) in relation to their in vitro antimalarial, antischistosomicidal, leishmanicidal and anti-hemolytic effects. The extracts exhibited antischistosomicidal (ED50 values from 418.4 to >1000.0 µg/mL) and antimalarial activities (IC50 values from 24.2 to 240.8 µg/mL) for both W2 and 3D7 strains in all intra-erythrocytic stages. Correlation analysis showed that the toxic effects may mainly be attributed to methylvescalagin (r = −0.548 to −0.951, p < 0.05) and 2,4-dihydroxybenzoic acid (r = −0.612 to −0.917, p < 0.05) contents. Moreover, the anti-hemolytic effect was associated to methylvescalagin (r = −0.597, p < 0.05). No toxic effects were observed for leishmaniasis and IMR90 normal cells. Herein, methylvescalagin was the bioactive compound of greatest interest once it presented simultaneous relation with antiparasitic and anti-hemolytic activities.
AB - Parasitic diseases have attracted worldwide attention of their consequent impact on mortality and morbidity. Accordingly, several plants have been screened for antiparasitic activity aiming to create new alternatives for treatment. These diseases have been neglected and have not attracted worldwide attention (nowadays), the health concerns are focused in chronic diseases, but it is necessary to focus on parasitic diseases and look for prophylactic alternatives, such as plant extracts. Although camu-camu (Myrciaria dubia) seeds are a rich source of antioxidant antimutagenic, cytotoxic, anti-inflammatory, antimicrobial, antihypertensive and neuroprotective compounds, nothing is known about their antiparasitic effects. Thus, in the present study we aimed to evaluate five extracts of camu-camu seeds (100% water, 100% ethyl alcohol, 50% water + 50% ethyl alcohol, 25% water + 75% ethyl alcohol, and 75% water + 25% ethyl alcohol) in relation to their in vitro antimalarial, antischistosomicidal, leishmanicidal and anti-hemolytic effects. The extracts exhibited antischistosomicidal (ED50 values from 418.4 to >1000.0 µg/mL) and antimalarial activities (IC50 values from 24.2 to 240.8 µg/mL) for both W2 and 3D7 strains in all intra-erythrocytic stages. Correlation analysis showed that the toxic effects may mainly be attributed to methylvescalagin (r = −0.548 to −0.951, p < 0.05) and 2,4-dihydroxybenzoic acid (r = −0.612 to −0.917, p < 0.05) contents. Moreover, the anti-hemolytic effect was associated to methylvescalagin (r = −0.597, p < 0.05). No toxic effects were observed for leishmaniasis and IMR90 normal cells. Herein, methylvescalagin was the bioactive compound of greatest interest once it presented simultaneous relation with antiparasitic and anti-hemolytic activities.
KW - Anti-hemolytic activity
KW - Antiparasitic
KW - Hydroalcoholic extract
KW - Methylvescalagin
KW - Myrciaria dubia
KW - Parasitic diseases
UR - http://www.scopus.com/inward/record.url?scp=85085259200&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2020.109334
DO - 10.1016/j.foodres.2020.109334
M3 - Article
C2 - 32846532
AN - SCOPUS:85085259200
SN - 0963-9969
VL - 136
JO - Food Research International
JF - Food Research International
M1 - 109334
ER -