Cannabinoid receptor agonist WIN 55,212-2 inhibits rat cortical dialysate gamma-aminobutyric acid levels

William T O'Connor; Luca Ferraro; Maria Cristina Tomasini; Tommaso Cassano; Berta Wonjie Bebe; Anna Siniscalchi; Pamela Magee; Sergio Tanganelli; Vincenzo Cuomo; Tiziana Antonelli

doi:10.1002/jnr.1224

Cannabinoid receptor agonist WIN 55,212-2 inhibits rat cortical dialysate gamma-aminobutyric acid levels

William T O'Connor, Luca Ferraro, Maria Cristina Tomasini, Tommaso Cassano, Berta Wonjie Bebe, Anna Siniscalchi, Pamela Magee, Sergio Tanganelli, Vincenzo Cuomo, Tiziana Antonelli

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of the cannabinoid receptor agonist WIN 55,212-2 (0.1-5 mg/kg i.p.) on endogenous extracellular γ-aminobutyric acid (GABA) levels in the cerebral cortex of the awake rat was investigated by using microdialysis. WIN 55,212-2 (1 and 5 mg/kg i.p.) was associated with a concentration-dependent decrease in dialysate GABA levels (-16%±4% and -26%±4% of basal values, respectively). The WIN 55,212-2 (5 mg/kg i.p.) induced-inhibition was counteracted by a dose (0.1 mg/kg i.p.) of the CB₁ receptor antagonist SR141716A, which by itself was without effect on cortical GABA levels. These findings suggest that cannabinoids decrease cortical GABA levels in vivo, an action that might underlie some of the cognitive and behavioral effects of acute exposure to marijuana.

Original language	English (Ireland)
Pages (from-to)	298-302
Number of pages	5
Journal	Journal of Neuroscience Research
Volume	66
Issue number	2
DOIs	https://doi.org/10.1002/jnr.1224
Publication status	Published - 15 Oct 2001

Keywords

Cannabinoid receptor agonist and antagonist
Intraperitoneal injection
Marijuana
Microdialysis

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10.1002/jnr.1224

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title = "Cannabinoid receptor agonist WIN 55,212-2 inhibits rat cortical dialysate gamma-aminobutyric acid levels",

abstract = "The effects of the cannabinoid receptor agonist WIN 55,212-2 (0.1-5 mg/kg i.p.) on endogenous extracellular γ-aminobutyric acid (GABA) levels in the cerebral cortex of the awake rat was investigated by using microdialysis. WIN 55,212-2 (1 and 5 mg/kg i.p.) was associated with a concentration-dependent decrease in dialysate GABA levels (-16%±4% and -26%±4% of basal values, respectively). The WIN 55,212-2 (5 mg/kg i.p.) induced-inhibition was counteracted by a dose (0.1 mg/kg i.p.) of the CB1 receptor antagonist SR141716A, which by itself was without effect on cortical GABA levels. These findings suggest that cannabinoids decrease cortical GABA levels in vivo, an action that might underlie some of the cognitive and behavioral effects of acute exposure to marijuana.",

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AU - O'Connor, William T

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AU - Tomasini, Maria Cristina

AU - Cassano, Tommaso

AU - Bebe, Berta Wonjie

AU - Siniscalchi, Anna

AU - Magee, Pamela

AU - Tanganelli, Sergio

AU - Cuomo, Vincenzo

AU - Antonelli, Tiziana

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N2 - The effects of the cannabinoid receptor agonist WIN 55,212-2 (0.1-5 mg/kg i.p.) on endogenous extracellular γ-aminobutyric acid (GABA) levels in the cerebral cortex of the awake rat was investigated by using microdialysis. WIN 55,212-2 (1 and 5 mg/kg i.p.) was associated with a concentration-dependent decrease in dialysate GABA levels (-16%±4% and -26%±4% of basal values, respectively). The WIN 55,212-2 (5 mg/kg i.p.) induced-inhibition was counteracted by a dose (0.1 mg/kg i.p.) of the CB1 receptor antagonist SR141716A, which by itself was without effect on cortical GABA levels. These findings suggest that cannabinoids decrease cortical GABA levels in vivo, an action that might underlie some of the cognitive and behavioral effects of acute exposure to marijuana.

AB - The effects of the cannabinoid receptor agonist WIN 55,212-2 (0.1-5 mg/kg i.p.) on endogenous extracellular γ-aminobutyric acid (GABA) levels in the cerebral cortex of the awake rat was investigated by using microdialysis. WIN 55,212-2 (1 and 5 mg/kg i.p.) was associated with a concentration-dependent decrease in dialysate GABA levels (-16%±4% and -26%±4% of basal values, respectively). The WIN 55,212-2 (5 mg/kg i.p.) induced-inhibition was counteracted by a dose (0.1 mg/kg i.p.) of the CB1 receptor antagonist SR141716A, which by itself was without effect on cortical GABA levels. These findings suggest that cannabinoids decrease cortical GABA levels in vivo, an action that might underlie some of the cognitive and behavioral effects of acute exposure to marijuana.

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Cannabinoid receptor agonist WIN 55,212-2 inhibits rat cortical dialysate gamma-aminobutyric acid levels

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Keywords

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