Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction

George Emílio Sampaio Barreto, Gleide Souza Dos Santos, Eryvaldo Sócrates Tabosa Egito, Ramon Dos Santos El-Bachá

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The aim of this work was to investigate the hypothesis that catechol inhibits FADH-linked basal respiration in mitochondria isolated from rat liver homogenates. Moreover, catechol ability to induce peroxidation of biomolecules in liver nuclear fractions was also studied. Methods: Rat liver homogenates were incubated with 1 mM 1,2-dihydroxybenzene (catechol) at pH 7.4 for up to 30 minutes. After that, mitochondrial fractions were isolated by differential centrifugation. Basal oxygen uptake was measured using a Clark-type electrode after the addition of 10 mM sodium succinate. Nuclear fractions were incubated in the presence of 1 mM catechol for 17 hours at room temperature and the peroxidation of biomolecules was investigated by the reaction with thiobarbituric acid, which was determined spectrophotometrically at 535 nm. Results: Catechol induced a time-dependent partial inhibition of FADH 2-linked basal mitochondrial respiration, however this substance was unable to induce a direct peroxidation of biomolecules in hepatic nuclear fractions. Conclusion: Catechol produced an inhibition of basal respiration associated to FADH2 in isolated liver mitochondria that could lead to cytotoxicity, ROS generation and cell death.

Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalActa cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia
Volume20
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2005
Externally publishedYes

Keywords

  • Catechol
  • Mitochondrial respiration
  • Peroxidation

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