TY - JOUR
T1 - Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
AU - Sampaio Barreto, George Emílio
AU - Souza Dos Santos, Gleide
AU - Tabosa Egito, Eryvaldo Sócrates
AU - Dos Santos El-Bachá, Ramon
PY - 2005
Y1 - 2005
N2 - Purpose: The aim of this work was to investigate the hypothesis that catechol inhibits FADH-linked basal respiration in mitochondria isolated from rat liver homogenates. Moreover, catechol ability to induce peroxidation of biomolecules in liver nuclear fractions was also studied. Methods: Rat liver homogenates were incubated with 1 mM 1,2-dihydroxybenzene (catechol) at pH 7.4 for up to 30 minutes. After that, mitochondrial fractions were isolated by differential centrifugation. Basal oxygen uptake was measured using a Clark-type electrode after the addition of 10 mM sodium succinate. Nuclear fractions were incubated in the presence of 1 mM catechol for 17 hours at room temperature and the peroxidation of biomolecules was investigated by the reaction with thiobarbituric acid, which was determined spectrophotometrically at 535 nm. Results: Catechol induced a time-dependent partial inhibition of FADH 2-linked basal mitochondrial respiration, however this substance was unable to induce a direct peroxidation of biomolecules in hepatic nuclear fractions. Conclusion: Catechol produced an inhibition of basal respiration associated to FADH2 in isolated liver mitochondria that could lead to cytotoxicity, ROS generation and cell death.
AB - Purpose: The aim of this work was to investigate the hypothesis that catechol inhibits FADH-linked basal respiration in mitochondria isolated from rat liver homogenates. Moreover, catechol ability to induce peroxidation of biomolecules in liver nuclear fractions was also studied. Methods: Rat liver homogenates were incubated with 1 mM 1,2-dihydroxybenzene (catechol) at pH 7.4 for up to 30 minutes. After that, mitochondrial fractions were isolated by differential centrifugation. Basal oxygen uptake was measured using a Clark-type electrode after the addition of 10 mM sodium succinate. Nuclear fractions were incubated in the presence of 1 mM catechol for 17 hours at room temperature and the peroxidation of biomolecules was investigated by the reaction with thiobarbituric acid, which was determined spectrophotometrically at 535 nm. Results: Catechol induced a time-dependent partial inhibition of FADH 2-linked basal mitochondrial respiration, however this substance was unable to induce a direct peroxidation of biomolecules in hepatic nuclear fractions. Conclusion: Catechol produced an inhibition of basal respiration associated to FADH2 in isolated liver mitochondria that could lead to cytotoxicity, ROS generation and cell death.
KW - Catechol
KW - Mitochondrial respiration
KW - Peroxidation
UR - http://www.scopus.com/inward/record.url?scp=55449122523&partnerID=8YFLogxK
U2 - 10.1590/s0102-86502005000700008
DO - 10.1590/s0102-86502005000700008
M3 - Article
AN - SCOPUS:55449122523
SN - 0102-8650
VL - 20
SP - 40
EP - 45
JO - Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia
JF - Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia
IS - SUPPL. 1
ER -