TY - JOUR
T1 - Cellular and Molecular Consequences of Stallion Sperm Cryopreservation
T2 - Recent Approaches to Improve Sperm Survival
AU - Contreras, María José
AU - Arias, María Elena
AU - Fuentes, Fernanda
AU - Muñoz, Erwin
AU - Bernecic, Naomi
AU - Fair, Sean
AU - Felmer, Ricardo
N1 - Publisher Copyright:
© 2023 Elsevier Inc.
PY - 2023/7
Y1 - 2023/7
N2 - Cryopreservation of stallion semen does not achieve the post-thaw quality or fertility results observed in other species like cattle. There are many reasons for this, but the membrane composition and intracellular changes in stallion sperm predispose them to low resistance to the cooling, freezing, and subsequent thawing process. Damage to the sperm results from different processes activated during cryopreservation, including oxidative stress, apoptosis, and structural modifications in the sperm membrane that increase the deleterious effect on sperm. In addition, significant individual variability is observed among stallions in the ability of sperm to survive the freeze-thaw process. Recent advances in genomics, transcriptomics, proteomics, metabolomics, and epigenetics are making it possible to advance our understanding of the cellular and molecular processes involved in the cryopreservation process, opening new possibilities for improvement. This review addresses the ongoing research on stallion semen cryopreservation, focusing on the cellular and molecular consequences of this procedure in stallions and discusses the new tools currently available to increase the tolerance of equine spermatozoa to freeze-thaw.
AB - Cryopreservation of stallion semen does not achieve the post-thaw quality or fertility results observed in other species like cattle. There are many reasons for this, but the membrane composition and intracellular changes in stallion sperm predispose them to low resistance to the cooling, freezing, and subsequent thawing process. Damage to the sperm results from different processes activated during cryopreservation, including oxidative stress, apoptosis, and structural modifications in the sperm membrane that increase the deleterious effect on sperm. In addition, significant individual variability is observed among stallions in the ability of sperm to survive the freeze-thaw process. Recent advances in genomics, transcriptomics, proteomics, metabolomics, and epigenetics are making it possible to advance our understanding of the cellular and molecular processes involved in the cryopreservation process, opening new possibilities for improvement. This review addresses the ongoing research on stallion semen cryopreservation, focusing on the cellular and molecular consequences of this procedure in stallions and discusses the new tools currently available to increase the tolerance of equine spermatozoa to freeze-thaw.
KW - Cryopreservation
KW - Freeze
KW - Plasma Membrane
KW - Semen
KW - Stallion
UR - http://www.scopus.com/inward/record.url?scp=85159435605&partnerID=8YFLogxK
U2 - 10.1016/j.jevs.2023.104499
DO - 10.1016/j.jevs.2023.104499
M3 - Review article
C2 - 37105416
AN - SCOPUS:85159435605
SN - 0737-0806
VL - 126
SP - 104499
JO - Journal of Equine Veterinary Science
JF - Journal of Equine Veterinary Science
M1 - 104499
ER -