Characteristics of protein partitioning in an aqueous micellar-gel system

L. J.P. Van Den Broeke; D. Van Roosmalen; M. P.J. Dohmen-Speelmans; C. H.J.T. Dietz; L. A.M. Van Der Wielen; J. T.F. Keurentjes

doi:10.1002/bit.20710

Characteristics of protein partitioning in an aqueous micellar-gel system

L. J.P. Van Den Broeke, D. Van Roosmalen, M. P.J. Dohmen-Speelmans, C. H.J.T. Dietz, L. A.M. Van Der Wielen, J. T.F. Keurentjes

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Abstract

Partitioning of proteins has been studied experimentally in a system combining a gel-bead phase and a nonionic micellar phase. The micellar phase consists of cylindrically shaped micelles, which are completely excluded from the gel-bead phase. Partitioning of single-component protein solutions (myoglobin, ovalbumin, and BSA) is determined by excluded-volume interactions in the micellar phase, and as a result the proteins prefer the gel-bead phase to the micellar phase. The protein concentration inside the gel beads increases with an increase in volume fraction of the micelles and increases with an increase in the size of the proteins. The protein partition coefficients obtained for a binary mixture of myoglobin and bovine serum albumin (BSA) show the same protein concentration dependence as the single-component protein partition coefficients.

Original language	English
Pages (from-to)	355-360
Number of pages	6
Journal	Biotechnology and Bioengineering
Volume	93
Issue number	2
DOIs	https://doi.org/10.1002/bit.20710
Publication status	Published - 5 Feb 2006
Externally published	Yes

Keywords

Aqueous micellar-gel systems
Excluded-volume interactions
Nonionic surfactant
Protein purification
Sephacryl large pore gel
Size exclusion

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title = "Characteristics of protein partitioning in an aqueous micellar-gel system",

abstract = "Partitioning of proteins has been studied experimentally in a system combining a gel-bead phase and a nonionic micellar phase. The micellar phase consists of cylindrically shaped micelles, which are completely excluded from the gel-bead phase. Partitioning of single-component protein solutions (myoglobin, ovalbumin, and BSA) is determined by excluded-volume interactions in the micellar phase, and as a result the proteins prefer the gel-bead phase to the micellar phase. The protein concentration inside the gel beads increases with an increase in volume fraction of the micelles and increases with an increase in the size of the proteins. The protein partition coefficients obtained for a binary mixture of myoglobin and bovine serum albumin (BSA) show the same protein concentration dependence as the single-component protein partition coefficients.",

keywords = "Aqueous micellar-gel systems, Excluded-volume interactions, Nonionic surfactant, Protein purification, Sephacryl large pore gel, Size exclusion",

author = "{Van Den Broeke}, {L. J.P.} and {Van Roosmalen}, D. and Dohmen-Speelmans, {M. P.J.} and Dietz, {C. H.J.T.} and {Van Der Wielen}, {L. A.M.} and Keurentjes, {J. T.F.}",

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AU - Van Den Broeke, L. J.P.

AU - Van Roosmalen, D.

AU - Dohmen-Speelmans, M. P.J.

AU - Dietz, C. H.J.T.

AU - Van Der Wielen, L. A.M.

AU - Keurentjes, J. T.F.

PY - 2006/2/5

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N2 - Partitioning of proteins has been studied experimentally in a system combining a gel-bead phase and a nonionic micellar phase. The micellar phase consists of cylindrically shaped micelles, which are completely excluded from the gel-bead phase. Partitioning of single-component protein solutions (myoglobin, ovalbumin, and BSA) is determined by excluded-volume interactions in the micellar phase, and as a result the proteins prefer the gel-bead phase to the micellar phase. The protein concentration inside the gel beads increases with an increase in volume fraction of the micelles and increases with an increase in the size of the proteins. The protein partition coefficients obtained for a binary mixture of myoglobin and bovine serum albumin (BSA) show the same protein concentration dependence as the single-component protein partition coefficients.

AB - Partitioning of proteins has been studied experimentally in a system combining a gel-bead phase and a nonionic micellar phase. The micellar phase consists of cylindrically shaped micelles, which are completely excluded from the gel-bead phase. Partitioning of single-component protein solutions (myoglobin, ovalbumin, and BSA) is determined by excluded-volume interactions in the micellar phase, and as a result the proteins prefer the gel-bead phase to the micellar phase. The protein concentration inside the gel beads increases with an increase in volume fraction of the micelles and increases with an increase in the size of the proteins. The protein partition coefficients obtained for a binary mixture of myoglobin and bovine serum albumin (BSA) show the same protein concentration dependence as the single-component protein partition coefficients.

KW - Aqueous micellar-gel systems

KW - Excluded-volume interactions

KW - Nonionic surfactant

KW - Protein purification

KW - Sephacryl large pore gel

KW - Size exclusion

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ER -

Characteristics of protein partitioning in an aqueous micellar-gel system

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Keywords

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