TY - JOUR
T1 - Chemical composition of a supercritical fluid (Sfe-CO2) extract from baeckea frutescens L. leaves and its bioactivity against two pathogenic fungi isolated from the tea plant (camellia sinensis (L.) O. Kuntze)
AU - Jiang, Hao
AU - Zhang, Mengting
AU - Qin, Li
AU - Wang, Dongxu
AU - Yu, Feng
AU - Liang, Wenhui
AU - Song, Chuankui
AU - Granato, Daniel
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/9
Y1 - 2020/9
N2 - Colletotrichum gloeosporioides and Pseudopestalotiopsis camelliae-sinensis are the two most important tea plant (Camellia sinensis L.) pathogenic fungi. Interest in natural plant extracts as alternatives to synthetic chemical fungicides to control plant pathogens is growing. In this study, the volatile fraction of Baeckea frutescens L. was extracted by supercritical fluid extraction (SFE-CO2), and its chemical composition was analyzed, and investigated for its antifungal activity against C. gloeosporioides and P. camelliae. The major constituents of the volatile fraction were β-caryophyllene (28.05%), α-caryophyllene (24.02%), δ-cadinene (6.29%) and eucalyptol (5.46%) in B. frutescens SFE-CO2 extracts. The terpineol, linalool, terpinen-4-ol and eucalyptol showed strong contact antifungal activity against P. camelliae and C. gloeosporioides with median inhibitory concentration (MIC50) in the range of 0.69 µL/mL to 2.79 µL/mL and 0.62 µL/mL to 2.18 µL/mL, respectively. Additionally, the volatile fraction had high fumigation antifungal activity against P. camelliae and C. gloeosporioides with an inhibition rate between 20.87% and 92.91%. Terpineol presented the highest antifungal activity in the contact and fumigation toxicity assays. Terpineol, linalool, terpinen-4-ol and eucalyptol were associated with the most active chemical compounds in the volatile fraction against the fungi. The results suggest that B. frutescens SFE-CO2 extracts are potential ingredients to develop a natural fungicide for control of tea plant pathogens.
AB - Colletotrichum gloeosporioides and Pseudopestalotiopsis camelliae-sinensis are the two most important tea plant (Camellia sinensis L.) pathogenic fungi. Interest in natural plant extracts as alternatives to synthetic chemical fungicides to control plant pathogens is growing. In this study, the volatile fraction of Baeckea frutescens L. was extracted by supercritical fluid extraction (SFE-CO2), and its chemical composition was analyzed, and investigated for its antifungal activity against C. gloeosporioides and P. camelliae. The major constituents of the volatile fraction were β-caryophyllene (28.05%), α-caryophyllene (24.02%), δ-cadinene (6.29%) and eucalyptol (5.46%) in B. frutescens SFE-CO2 extracts. The terpineol, linalool, terpinen-4-ol and eucalyptol showed strong contact antifungal activity against P. camelliae and C. gloeosporioides with median inhibitory concentration (MIC50) in the range of 0.69 µL/mL to 2.79 µL/mL and 0.62 µL/mL to 2.18 µL/mL, respectively. Additionally, the volatile fraction had high fumigation antifungal activity against P. camelliae and C. gloeosporioides with an inhibition rate between 20.87% and 92.91%. Terpineol presented the highest antifungal activity in the contact and fumigation toxicity assays. Terpineol, linalool, terpinen-4-ol and eucalyptol were associated with the most active chemical compounds in the volatile fraction against the fungi. The results suggest that B. frutescens SFE-CO2 extracts are potential ingredients to develop a natural fungicide for control of tea plant pathogens.
KW - Antifungal activity
KW - Baeckea frutescens
KW - Camellia sinensis
KW - Colletotrichum gloeosporioides
KW - Pathogenic fungi
KW - Pseudopestalotiopsis camelliae-sinensis
KW - Supercritical fluid extraction
KW - Tea tree plant diseases
UR - http://www.scopus.com/inward/record.url?scp=85093894901&partnerID=8YFLogxK
U2 - 10.3390/plants9091119
DO - 10.3390/plants9091119
M3 - Article
AN - SCOPUS:85093894901
VL - 9
SP - 1
EP - 15
JO - Plants
JF - Plants
IS - 9
M1 - 1119
ER -