Chronocoulometric determination of urea in human serum using an inkjet printed biosensor

Suman, Emmet O'Reilly, Michele Kelly, Aoife Morrin, Malcolm R. Smyth, Anthony J. Killard

Research output: Contribution to journalArticlepeer-review

Abstract

A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100mM. Urea could be measured by the sensor in the range of 2-12mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6mM urea, the developed sensor had an average positive experimental bias of 0.12mM (<2% RSD) over the reference method.

Original languageEnglish
Pages (from-to)98-102
Number of pages5
JournalAnalytica Chimica Acta
Volume697
Issue number1-2
DOIs
Publication statusPublished - 4 Jul 2011
Externally publishedYes

Keywords

  • Chronocoulometry
  • Inkjet
  • Nanoparticle
  • Polyaniline
  • Urea

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