Chronocoulometric determination of urea in human serum using an inkjet printed biosensor

Suman; Emmet O'Reilly; Michele Kelly; Aoife Morrin; Malcolm R. Smyth; Anthony J. Killard

doi:10.1016/j.aca.2011.04.036

Chronocoulometric determination of urea in human serum using an inkjet printed biosensor

Suman, Emmet O'Reilly, Michele Kelly, Aoife Morrin, Malcolm R. Smyth, Anthony J. Killard

Research output: Contribution to journal › Article › peer-review

Abstract

A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100mM. Urea could be measured by the sensor in the range of 2-12mM (r²=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6mM urea, the developed sensor had an average positive experimental bias of 0.12mM (<2% RSD) over the reference method.

Original language	English
Pages (from-to)	98-102
Number of pages	5
Journal	Analytica Chimica Acta
Volume	697
Issue number	1-2
DOIs	https://doi.org/10.1016/j.aca.2011.04.036
Publication status	Published - 4 Jul 2011
Externally published	Yes

Keywords

Chronocoulometry
Inkjet
Nanoparticle
Polyaniline
Urea

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10.1016/j.aca.2011.04.036

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title = "Chronocoulometric determination of urea in human serum using an inkjet printed biosensor",

abstract = "A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100mM. Urea could be measured by the sensor in the range of 2-12mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6mM urea, the developed sensor had an average positive experimental bias of 0.12mM (<2\% RSD) over the reference method.",

keywords = "Chronocoulometry, Inkjet, Nanoparticle, Polyaniline, Urea",

author = "Suman and Emmet O'Reilly and Michele Kelly and Aoife Morrin and Smyth, \{Malcolm R.\} and Killard, \{Anthony J.\}",

year = "2011",

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doi = "10.1016/j.aca.2011.04.036",

language = "English",

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journal = "Analytica Chimica Acta",

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AU - Suman,

AU - O'Reilly, Emmet

AU - Kelly, Michele

AU - Morrin, Aoife

AU - Smyth, Malcolm R.

AU - Killard, Anthony J.

PY - 2011/7/4

Y1 - 2011/7/4

N2 - A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100mM. Urea could be measured by the sensor in the range of 2-12mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6mM urea, the developed sensor had an average positive experimental bias of 0.12mM (<2% RSD) over the reference method.

AB - A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100mM. Urea could be measured by the sensor in the range of 2-12mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6mM urea, the developed sensor had an average positive experimental bias of 0.12mM (<2% RSD) over the reference method.

KW - Chronocoulometry

KW - Inkjet

KW - Nanoparticle

KW - Polyaniline

KW - Urea

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ER -

Chronocoulometric determination of urea in human serum using an inkjet printed biosensor

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Keywords

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