Clitoria ternatea L. petal bioactive compounds display antioxidant, antihemolytic and antihypertensive effects, inhibit α-amylase and α-glucosidase activities and reduce human LDL cholesterol and DNA induced oxidation

Graziela Bragueto Escher, Mariza Boscacci Marques, Mariana Araújo Vieira do Carmo, Luciana Azevedo, Marianna Miranda Furtado, Anderson S. Sant'Ana, Marcia Cristina da Silva, Maria Inês Genovese, Mingchun Wen, Liang Zhang, Won Young Oh, Fereidoon Shahidi, Neiva Deliberali Rosso, Daniel Granato

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to use a statistical approach to optimise the experimental conditions regarding the extraction of bioactive compounds, and to analyse the in vitro functional properties of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of Clitoria ternatea petals. The results showed that the factors of temperature and time influenced the extraction of phenolic compounds, antioxidant activity and the physicochemical parameters. Simultaneous optimisation showed that the same levels of bioactive compounds were extracted when using temperatures from 11.7 to 68.3 °C and times from 8.47 to 51.12 min. Principal component analysis revealed the experimental conditions that provided the extraction producing the highest level of phenolic content (40 °C/30 min). The CLE showed antimicrobial activity; protective effect against hemolysis of erythrocytes; inhibition of α-amylase, α-glucosidase and angiotensin-I-converting (ACE-I) enzymes; and inhibition of lipid peroxidation. The CLE and PPE demonstrated oxygen radical absorption capacity; inhibition of DNA strand scission; inhibition of LDL cholesterol oxidation; intracellular antioxidant activity against reactive oxygen species (>100 μg/mL); and no cytotoxicity (IC50, GI50 and LC50 > 900 μg/mL) against A549, HCT8 and IMR90 cell lines.

Original languageEnglish
Article number108763
Pages (from-to)108763
JournalFood Research International
Volume128
DOIs
Publication statusPublished - Feb 2020
Externally publishedYes

Keywords

  • 2,4-Dihydroxybenzoic acid (PubChem CID: 1491)
  • Anthocyanins
  • Antihypertensive activity
  • Caffeic acid (PubChem CID: 689043)
  • Delphinidin-3-O-glucoside (PubChem CID: 443650)
  • Gallic acid (PubChem CID: 370)
  • Inhibitor of DNA strand scission
  • p-Coumaric acid (PubChem CID: 637542)
  • Principal component analysis
  • Procyanidin A2 (PubChem CID: 124025)
  • Protocatechuic acid (PubChem CID: 72)
  • Quercetin-3-rutinoside (PubChem CID: 5280805)
  • Response surface methodology
  • Syringic acid (PubChem CID: 10742)
  • Unconventional foods

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