Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D

Fan Yang; Seung Woo Cho; Sun Mi Son; Sarah P. Hudson; Said Bogatyrev; Lily Keung; Daniel S. Kohane; Robert Langer; Daniel G. Anderson

doi:10.1021/bm100357t

Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D

Fan Yang, Seung Woo Cho, Sun Mi Son, Sarah P. Hudson, Said Bogatyrev, Lily Keung, Daniel S. Kohane, Robert Langer, Daniel G. Anderson

Research output: Contribution to journal › Article › peer-review

Abstract

Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.

Original language	English
Pages (from-to)	1909-1914
Number of pages	6
Journal	Biomacromolecules
Volume	11
Issue number	8
DOIs	https://doi.org/10.1021/bm100357t
Publication status	Published - 9 Aug 2010
Externally published	Yes

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title = "Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D",

abstract = "Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.",

author = "Fan Yang and Cho, {Seung Woo} and Son, {Sun Mi} and Hudson, {Sarah P.} and Said Bogatyrev and Lily Keung and Kohane, {Daniel S.} and Robert Langer and Anderson, {Daniel G.}",

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doi = "10.1021/bm100357t",

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T1 - Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D

AU - Yang, Fan

AU - Cho, Seung Woo

AU - Son, Sun Mi

AU - Hudson, Sarah P.

AU - Bogatyrev, Said

AU - Keung, Lily

AU - Kohane, Daniel S.

AU - Langer, Robert

AU - Anderson, Daniel G.

PY - 2010/8/9

Y1 - 2010/8/9

N2 - Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.

AB - Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.

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Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D

Abstract

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