TY - JOUR
T1 - Comparison of mesoporous silicate supports for the immobilisation and activity of cytochrome c and lipase
AU - Abdallah, Noreldeen H.
AU - Schlumpberger, Miriam
AU - Gaffney, Darragh A.
AU - Hanrahan, John P.
AU - Tobin, Joseph M.
AU - Magner, Edmond
PY - 2014/10
Y1 - 2014/10
N2 - The activity and stability of Candida antartica lipase B (CALB) and cytochrome c immobilised onto SBA-15 and a porous spherical silicate material (PPS) were examined. The materials possess similar pore diameters but have different morphologies, pore volumes and surface areas. CALB exhibited higher catalytic activity and stability on SBA-15 when compared to PPS, while cytochrome c showed similar catalytic activity on both materials. The activity of CALB immobilised on SBA-15 was retained (95%) after 7 uses, while CALB immobilised on PPS retained only 43% activity. Such changes can be mainly ascribed to the different physical properties (pore volume, surface area and pore shape) of the supports.
AB - The activity and stability of Candida antartica lipase B (CALB) and cytochrome c immobilised onto SBA-15 and a porous spherical silicate material (PPS) were examined. The materials possess similar pore diameters but have different morphologies, pore volumes and surface areas. CALB exhibited higher catalytic activity and stability on SBA-15 when compared to PPS, while cytochrome c showed similar catalytic activity on both materials. The activity of CALB immobilised on SBA-15 was retained (95%) after 7 uses, while CALB immobilised on PPS retained only 43% activity. Such changes can be mainly ascribed to the different physical properties (pore volume, surface area and pore shape) of the supports.
KW - Cytochrome c
KW - Immobilisation
KW - Lipase
KW - Mesoporous silicate
UR - http://www.scopus.com/inward/record.url?scp=84905192116&partnerID=8YFLogxK
U2 - 10.1016/j.molcatb.2014.06.007
DO - 10.1016/j.molcatb.2014.06.007
M3 - Article
AN - SCOPUS:84905192116
SN - 1381-1177
VL - 108
SP - 82
EP - 88
JO - Journal of Molecular Catalysis B: Enzymatic
JF - Journal of Molecular Catalysis B: Enzymatic
ER -