Computational modeling and preliminary iroN, fepA, and cirA gene expression in Salmonella Enteritidis under iron-deficiency-induced conditions

Lina J. Zárate-Bonilla, Patricia Del Portillo, Homero Sáenz-Suárez, Janneth Gonzáles-Santos, George E. Barreto-Sampaio, Raúl A. Poutou-Piñales, Andrés Felipe Rey, Jairo Guillermo Rey

Research output: Contribution to journalArticlepeer-review

Abstract

Salmonellosis outbreaks in Europe, the United States, and Latin America have been associated with contaminated food derivatives including meat from the poultry industry. Salmonella grown under iron-limiting conditions has the capability to increase concentration of several iron-regulated outer-membrane proteins to augment the acquisition of the metal. These proteins have been proved to have immunogenic properties. Our aim was to increase the relative expression of iroN, fepA, and cirA in Salmonella Enteritidis domestic strain. Furthermore, we proposed a 3-dimensional structure model for each protein to predict and locate antigenic peptides. Our eventual objective is to produce an effective vaccine against regional avian salmonellosis. Two simple factorial designs were carried out to discriminate between 2 nitrogen sources and determine chelating-agent addition timing to augment relative gene expression. Two antigenic peptides located at the external face of each protein and 2 typical domains of iron-regulated outer-membrane proteins, plug and TonB-dep-Rec, were identified from the 3-dimensional models. Tryptone was selected as the best nitrogen source based on growth rate (Ux = 0.36 h-1) and biomass productivity (Px = 0.9 g.h-1.L-1) as determined by a general factorial design. Optimum timing for chelating agent addition was in the middle of the log phase, which allowed relative expressions at 4 h of culture. Increase in iroN, fepA, and cirA relative expression was favored by the length of log phase and the addition of chelating agent, which decreased chelating toxicity and enhanced cell growth rate.

Original languageEnglish
Pages (from-to)221-230
Number of pages10
JournalPoultry Science
Volume93
Issue number1
DOIs
Publication statusPublished - Jan 2014
Externally publishedYes

Keywords

  • CirA
  • Computer modeling
  • FepA
  • IroN
  • Iron deficiency condition
  • Iron-regulated outer-membrane protein
  • Salmonella Enteritidis

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