TY - JOUR
T1 - Computational modeling and preliminary iroN, fepA, and cirA gene expression in Salmonella Enteritidis under iron-deficiency-induced conditions
AU - Zárate-Bonilla, Lina J.
AU - Del Portillo, Patricia
AU - Sáenz-Suárez, Homero
AU - Gonzáles-Santos, Janneth
AU - Barreto-Sampaio, George E.
AU - Poutou-Piñales, Raúl A.
AU - Rey, Andrés Felipe
AU - Rey, Jairo Guillermo
PY - 2014/1
Y1 - 2014/1
N2 - Salmonellosis outbreaks in Europe, the United States, and Latin America have been associated with contaminated food derivatives including meat from the poultry industry. Salmonella grown under iron-limiting conditions has the capability to increase concentration of several iron-regulated outer-membrane proteins to augment the acquisition of the metal. These proteins have been proved to have immunogenic properties. Our aim was to increase the relative expression of iroN, fepA, and cirA in Salmonella Enteritidis domestic strain. Furthermore, we proposed a 3-dimensional structure model for each protein to predict and locate antigenic peptides. Our eventual objective is to produce an effective vaccine against regional avian salmonellosis. Two simple factorial designs were carried out to discriminate between 2 nitrogen sources and determine chelating-agent addition timing to augment relative gene expression. Two antigenic peptides located at the external face of each protein and 2 typical domains of iron-regulated outer-membrane proteins, plug and TonB-dep-Rec, were identified from the 3-dimensional models. Tryptone was selected as the best nitrogen source based on growth rate (Ux = 0.36 h-1) and biomass productivity (Px = 0.9 g.h-1.L-1) as determined by a general factorial design. Optimum timing for chelating agent addition was in the middle of the log phase, which allowed relative expressions at 4 h of culture. Increase in iroN, fepA, and cirA relative expression was favored by the length of log phase and the addition of chelating agent, which decreased chelating toxicity and enhanced cell growth rate.
AB - Salmonellosis outbreaks in Europe, the United States, and Latin America have been associated with contaminated food derivatives including meat from the poultry industry. Salmonella grown under iron-limiting conditions has the capability to increase concentration of several iron-regulated outer-membrane proteins to augment the acquisition of the metal. These proteins have been proved to have immunogenic properties. Our aim was to increase the relative expression of iroN, fepA, and cirA in Salmonella Enteritidis domestic strain. Furthermore, we proposed a 3-dimensional structure model for each protein to predict and locate antigenic peptides. Our eventual objective is to produce an effective vaccine against regional avian salmonellosis. Two simple factorial designs were carried out to discriminate between 2 nitrogen sources and determine chelating-agent addition timing to augment relative gene expression. Two antigenic peptides located at the external face of each protein and 2 typical domains of iron-regulated outer-membrane proteins, plug and TonB-dep-Rec, were identified from the 3-dimensional models. Tryptone was selected as the best nitrogen source based on growth rate (Ux = 0.36 h-1) and biomass productivity (Px = 0.9 g.h-1.L-1) as determined by a general factorial design. Optimum timing for chelating agent addition was in the middle of the log phase, which allowed relative expressions at 4 h of culture. Increase in iroN, fepA, and cirA relative expression was favored by the length of log phase and the addition of chelating agent, which decreased chelating toxicity and enhanced cell growth rate.
KW - CirA
KW - Computer modeling
KW - FepA
KW - IroN
KW - Iron deficiency condition
KW - Iron-regulated outer-membrane protein
KW - Salmonella Enteritidis
UR - http://www.scopus.com/inward/record.url?scp=84896063639&partnerID=8YFLogxK
U2 - 10.3382/ps.2012-02993
DO - 10.3382/ps.2012-02993
M3 - Article
C2 - 24570443
AN - SCOPUS:84896063639
SN - 0032-5791
VL - 93
SP - 221
EP - 230
JO - Poultry Science
JF - Poultry Science
IS - 1
ER -