TY - GEN
T1 - Conducting polymer based DNA biosensor for the detection of the Bacillus cereus group species
AU - Velusamy, Vijayalakshmi
AU - Arshak, Khalil
AU - Korostynska, Olga
AU - Oliwa, Kamila
AU - Adley, Catherine
PY - 2009
Y1 - 2009
N2 - Biosensor designs are emerging at a significant rate and play an increasingly important role in foodborne pathogen detection. Conducting polymers are excellent tools for the fabrication of biosensors and polypyrrole has been used in the detection of biomolecules due to its unique properties. The prime intention of this paper was to pioneer the design and fabrication of a single-strand (ss) DNA biosensor for the detection of the Bacillus cereus (B.cereus) group species. Growth of B. cereus, results in production of several highly active toxins. Therefore, consumption of food containing >10 6 bacteria/gm may results in emetic and diarrhoeal syndromes. The most common source of this bacterium is foundin liquid food products, milk powder, mixed food products and is of particular concern in the baby formula industry. The electrochemical deposition technique, such as cyclic voltammetry, was used to develop and test a model DNA-based biosensor on a gold electrode electropolymerized with polypyrrole. The electrically conducting polymer, polypyrrole isused as a platform for immobilizing DNA (1μg) on the gold electrode surface, since it can be more easily deposited from neutral pH aqueous solutions of pyrrolemonomers. The average current peak during the electrodeposition event is 288μA. There is a clear change in the current after hybridization of the complementary oligonucleotide (6.35μA) and for the noncomplementary oligonucleotide (5.77μA). The drop in current after each event was clearly noticeable and it proved to be effective.
AB - Biosensor designs are emerging at a significant rate and play an increasingly important role in foodborne pathogen detection. Conducting polymers are excellent tools for the fabrication of biosensors and polypyrrole has been used in the detection of biomolecules due to its unique properties. The prime intention of this paper was to pioneer the design and fabrication of a single-strand (ss) DNA biosensor for the detection of the Bacillus cereus (B.cereus) group species. Growth of B. cereus, results in production of several highly active toxins. Therefore, consumption of food containing >10 6 bacteria/gm may results in emetic and diarrhoeal syndromes. The most common source of this bacterium is foundin liquid food products, milk powder, mixed food products and is of particular concern in the baby formula industry. The electrochemical deposition technique, such as cyclic voltammetry, was used to develop and test a model DNA-based biosensor on a gold electrode electropolymerized with polypyrrole. The electrically conducting polymer, polypyrrole isused as a platform for immobilizing DNA (1μg) on the gold electrode surface, since it can be more easily deposited from neutral pH aqueous solutions of pyrrolemonomers. The average current peak during the electrodeposition event is 288μA. There is a clear change in the current after hybridization of the complementary oligonucleotide (6.35μA) and for the noncomplementary oligonucleotide (5.77μA). The drop in current after each event was clearly noticeable and it proved to be effective.
KW - Bacillus cereus group spp
KW - Biosensor
KW - Conducting polymers
KW - Cyclic voltammetry
KW - DNA
KW - Foodborne pathogen detection
UR - http://www.scopus.com/inward/record.url?scp=69649103799&partnerID=8YFLogxK
U2 - 10.1117/12.818631
DO - 10.1117/12.818631
M3 - Conference contribution
AN - SCOPUS:69649103799
SN - 9780819475817
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Sensing for Agriculture and Food Quality and Safety
T2 - Sensing for Agriculture and Food Quality and Safety
Y2 - 14 April 2009 through 15 April 2009
ER -