Conducting polymer based DNA biosensor for the detection of the Bacillus cereus group species

Vijayalakshmi Velusamy, Khalil Arshak, Olga Korostynska, Kamila Oliwa, Catherine Adley

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Biosensor designs are emerging at a significant rate and play an increasingly important role in foodborne pathogen detection. Conducting polymers are excellent tools for the fabrication of biosensors and polypyrrole has been used in the detection of biomolecules due to its unique properties. The prime intention of this paper was to pioneer the design and fabrication of a single-strand (ss) DNA biosensor for the detection of the Bacillus cereus (B.cereus) group species. Growth of B. cereus, results in production of several highly active toxins. Therefore, consumption of food containing >10 6 bacteria/gm may results in emetic and diarrhoeal syndromes. The most common source of this bacterium is foundin liquid food products, milk powder, mixed food products and is of particular concern in the baby formula industry. The electrochemical deposition technique, such as cyclic voltammetry, was used to develop and test a model DNA-based biosensor on a gold electrode electropolymerized with polypyrrole. The electrically conducting polymer, polypyrrole isused as a platform for immobilizing DNA (1μg) on the gold electrode surface, since it can be more easily deposited from neutral pH aqueous solutions of pyrrolemonomers. The average current peak during the electrodeposition event is 288μA. There is a clear change in the current after hybridization of the complementary oligonucleotide (6.35μA) and for the noncomplementary oligonucleotide (5.77μA). The drop in current after each event was clearly noticeable and it proved to be effective.

Original languageEnglish
Title of host publicationSensing for Agriculture and Food Quality and Safety
DOIs
Publication statusPublished - 2009
EventSensing for Agriculture and Food Quality and Safety - Orlando, FL, United States
Duration: 14 Apr 200915 Apr 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7315
ISSN (Print)0277-786X

Conference

ConferenceSensing for Agriculture and Food Quality and Safety
Country/TerritoryUnited States
CityOrlando, FL
Period14/04/0915/04/09

Keywords

  • Bacillus cereus group spp
  • Biosensor
  • Conducting polymers
  • Cyclic voltammetry
  • DNA
  • Foodborne pathogen detection

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