Design of specific DNA primers to detect the Bacillus cereus group species

Catherine Adley, Khalil Arshak, Camila Molnar, Kamila Oliwa, Vijayalakshmi Velusamy

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

One of the most prevalent pathogens that cause foodborne outbreaks is the Bacillus cereus (B. cereus) group species (spp.) generally found in different types of food. Recently many researchers are focusing towards the progress of rapid methods to detect foodborne pathogens. Every year many innovative methodologies for bacterial detection are being developed to improve sensitivity and speed of detection. DNA biosensor is one of the proposed solutions for the detection of foodborne pathogens which can be used for food quality assurance. This paper reports that the DNA sequences named BCFomp1/BCRomp1 can be used for the specific detection of the B. cereus group spp. Analysis of these primers using standard PCR analysis showed that the minimum level of detection was 103 CFU/ml. The lowest number of bacterial cell per reaction tube that can be amplified was 5 CFU and the minimum quantity of DNA that can be amplified was found to be 1pg.

Original languageEnglish
Title of host publicationSAS 2009 - IEEE Sensors Applications Symposium Proceedings
Pages236-239
Number of pages4
DOIs
Publication statusPublished - 2009
EventIEEE Sensors Applications Symposium, SAS 2009 - New Orleans, LA, United States
Duration: 17 Feb 200919 Feb 2009

Publication series

NameSAS 2009 - IEEE Sensors Applications Symposium Proceedings

Conference

ConferenceIEEE Sensors Applications Symposium, SAS 2009
Country/TerritoryUnited States
CityNew Orleans, LA
Period17/02/0919/02/09

Keywords

  • Biosensor
  • DNA
  • Foodborne pathogen detection
  • PCR
  • Primers
  • The B. cereus group spp.

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