Design of specific DNA primers to detect the Bacillus cereus group species

Catherine Adley; Khalil Arshak; Camila Molnar; Kamila Oliwa; Vijayalakshmi Velusamy

doi:10.1109/SAS.2009.4801807

Design of specific DNA primers to detect the Bacillus cereus group species

Catherine Adley
, Khalil Arshak
, Camila Molnar
, Kamila Oliwa
, Vijayalakshmi Velusamy

University of Limerick

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

One of the most prevalent pathogens that cause foodborne outbreaks is the Bacillus cereus (B. cereus) group species (spp.) generally found in different types of food. Recently many researchers are focusing towards the progress of rapid methods to detect foodborne pathogens. Every year many innovative methodologies for bacterial detection are being developed to improve sensitivity and speed of detection. DNA biosensor is one of the proposed solutions for the detection of foodborne pathogens which can be used for food quality assurance. This paper reports that the DNA sequences named BCFomp1/BCRomp1 can be used for the specific detection of the B. cereus group spp. Analysis of these primers using standard PCR analysis showed that the minimum level of detection was 103 CFU/ml. The lowest number of bacterial cell per reaction tube that can be amplified was 5 CFU and the minimum quantity of DNA that can be amplified was found to be 1pg.

Original language	English
Title of host publication	SAS 2009 - IEEE Sensors Applications Symposium Proceedings
Pages	236-239
Number of pages	4
DOIs	https://doi.org/10.1109/SAS.2009.4801807
Publication status	Published - 2009
Event	IEEE Sensors Applications Symposium, SAS 2009 - New Orleans, LA, United States Duration: 17 Feb 2009 → 19 Feb 2009

Publication series

Name	SAS 2009 - IEEE Sensors Applications Symposium Proceedings

Conference

Conference	IEEE Sensors Applications Symposium, SAS 2009
Country/Territory	United States
City	New Orleans, LA
Period	17/02/09 → 19/02/09

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biosensor
DNA
Foodborne pathogen detection
PCR
Primers
The B. cereus group spp.

Access to Document

10.1109/SAS.2009.4801807

Cite this

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title = "Design of specific DNA primers to detect the Bacillus cereus group species",

abstract = "One of the most prevalent pathogens that cause foodborne outbreaks is the Bacillus cereus (B. cereus) group species (spp.) generally found in different types of food. Recently many researchers are focusing towards the progress of rapid methods to detect foodborne pathogens. Every year many innovative methodologies for bacterial detection are being developed to improve sensitivity and speed of detection. DNA biosensor is one of the proposed solutions for the detection of foodborne pathogens which can be used for food quality assurance. This paper reports that the DNA sequences named BCFomp1/BCRomp1 can be used for the specific detection of the B. cereus group spp. Analysis of these primers using standard PCR analysis showed that the minimum level of detection was 103 CFU/ml. The lowest number of bacterial cell per reaction tube that can be amplified was 5 CFU and the minimum quantity of DNA that can be amplified was found to be 1pg.",

keywords = "Biosensor, DNA, Foodborne pathogen detection, PCR, Primers, The B. cereus group spp.",

author = "Catherine Adley and Khalil Arshak and Camila Molnar and Kamila Oliwa and Vijayalakshmi Velusamy",

year = "2009",

doi = "10.1109/SAS.2009.4801807",

language = "English",

isbn = "9781424427871",

series = "SAS 2009 - IEEE Sensors Applications Symposium Proceedings",

pages = "236--239",

booktitle = "SAS 2009 - IEEE Sensors Applications Symposium Proceedings",

note = "IEEE Sensors Applications Symposium, SAS 2009 ; Conference date: 17-02-2009 Through 19-02-2009",

}

Adley, C, Arshak, K, Molnar, C, Oliwa, K & Velusamy, V 2009, Design of specific DNA primers to detect the Bacillus cereus group species. in SAS 2009 - IEEE Sensors Applications Symposium Proceedings., 4801807, SAS 2009 - IEEE Sensors Applications Symposium Proceedings, pp. 236-239, IEEE Sensors Applications Symposium, SAS 2009, New Orleans, LA, United States, 17/02/09. https://doi.org/10.1109/SAS.2009.4801807

Design of specific DNA primers to detect the Bacillus cereus group species. / Adley, Catherine; Arshak, Khalil; Molnar, Camila et al.
SAS 2009 - IEEE Sensors Applications Symposium Proceedings. 2009. p. 236-239 4801807 (SAS 2009 - IEEE Sensors Applications Symposium Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Design of specific DNA primers to detect the Bacillus cereus group species

AU - Adley, Catherine

AU - Arshak, Khalil

AU - Molnar, Camila

AU - Oliwa, Kamila

AU - Velusamy, Vijayalakshmi

PY - 2009

Y1 - 2009

N2 - One of the most prevalent pathogens that cause foodborne outbreaks is the Bacillus cereus (B. cereus) group species (spp.) generally found in different types of food. Recently many researchers are focusing towards the progress of rapid methods to detect foodborne pathogens. Every year many innovative methodologies for bacterial detection are being developed to improve sensitivity and speed of detection. DNA biosensor is one of the proposed solutions for the detection of foodborne pathogens which can be used for food quality assurance. This paper reports that the DNA sequences named BCFomp1/BCRomp1 can be used for the specific detection of the B. cereus group spp. Analysis of these primers using standard PCR analysis showed that the minimum level of detection was 103 CFU/ml. The lowest number of bacterial cell per reaction tube that can be amplified was 5 CFU and the minimum quantity of DNA that can be amplified was found to be 1pg.

AB - One of the most prevalent pathogens that cause foodborne outbreaks is the Bacillus cereus (B. cereus) group species (spp.) generally found in different types of food. Recently many researchers are focusing towards the progress of rapid methods to detect foodborne pathogens. Every year many innovative methodologies for bacterial detection are being developed to improve sensitivity and speed of detection. DNA biosensor is one of the proposed solutions for the detection of foodborne pathogens which can be used for food quality assurance. This paper reports that the DNA sequences named BCFomp1/BCRomp1 can be used for the specific detection of the B. cereus group spp. Analysis of these primers using standard PCR analysis showed that the minimum level of detection was 103 CFU/ml. The lowest number of bacterial cell per reaction tube that can be amplified was 5 CFU and the minimum quantity of DNA that can be amplified was found to be 1pg.

KW - Biosensor

KW - DNA

KW - Foodborne pathogen detection

KW - PCR

KW - Primers

KW - The B. cereus group spp.

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DO - 10.1109/SAS.2009.4801807

M3 - Conference contribution

AN - SCOPUS:65249128046

SN - 9781424427871

T3 - SAS 2009 - IEEE Sensors Applications Symposium Proceedings

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EP - 239

BT - SAS 2009 - IEEE Sensors Applications Symposium Proceedings

T2 - IEEE Sensors Applications Symposium, SAS 2009

Y2 - 17 February 2009 through 19 February 2009

ER -

Design of specific DNA primers to detect the Bacillus cereus group species

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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