TY - JOUR
T1 - Designing Functionalized Mesoporous Materials for Enzyme Immobilization
T2 - Locating Enzymes by Using Advanced TEM Techniques
AU - Mayoral, Alvaro
AU - Arenal, Raul
AU - Gascón, Victoria
AU - Márquez-Álvarez, Carlos
AU - Blanco, Rosa M.
AU - Díaz, Isabel
PY - 2013/4
Y1 - 2013/4
N2 - One of the widely accepted uses of ordered mesoporous materials is as supports of enzymes for biotechnological applications. Enzymes have been trapped, anchored, or encapsulated in organized porous networks of the mesoporous range (2-50nm). The reactivity of the surface of mesoporous materials has enabled the synthesis of various supports by using different forces for the immobilization process. To design catalysts for specific applications, we have developed functionalized mesoporous materials with tunable hydrophobicity for the immobilization of lipase. More recently, we moved to the immobilization of laccase with amino-functionalized ordered mesoporous materials. In this case, it is required to use pore expanders along with optimized functionalization techniques. Advanced TEM techniques have been applied to locate not only the functional groups but also the macromolecules inside the silica matrix.
AB - One of the widely accepted uses of ordered mesoporous materials is as supports of enzymes for biotechnological applications. Enzymes have been trapped, anchored, or encapsulated in organized porous networks of the mesoporous range (2-50nm). The reactivity of the surface of mesoporous materials has enabled the synthesis of various supports by using different forces for the immobilization process. To design catalysts for specific applications, we have developed functionalized mesoporous materials with tunable hydrophobicity for the immobilization of lipase. More recently, we moved to the immobilization of laccase with amino-functionalized ordered mesoporous materials. In this case, it is required to use pore expanders along with optimized functionalization techniques. Advanced TEM techniques have been applied to locate not only the functional groups but also the macromolecules inside the silica matrix.
KW - Electron microscopy
KW - Enzyme immobilization
KW - Enzymes
KW - Functionalized mesoporous materials
KW - Laccase
KW - Lipase
UR - http://www.scopus.com/inward/record.url?scp=84875575930&partnerID=8YFLogxK
U2 - 10.1002/cctc.201200737
DO - 10.1002/cctc.201200737
M3 - Article
AN - SCOPUS:84875575930
SN - 1867-3880
VL - 5
SP - 903
EP - 909
JO - ChemCatChem
JF - ChemCatChem
IS - 4
ER -