Diethylmaleate, a pro-oxidant, attenuates experimental ischaemia-reperfusion-induced lung injury

P. D. Kiely, J. C. Wang, C. J. Kelly, C. Condron, R. G.K. Watson, D. J. Bouchier-Hayes

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Systemic ischaemia-reperfusion (IR) injury is in part an oxidant injury mediated by neutrophils. Diethylmaleate (DEM), an intracellular pro-oxidant agent, has been shown to alleviate neutrophil-mediated tissue injury. The aim of this study was to evaluate whether DEM could have a protective effect on neutrophil-mediated lung injury in an animal model of lower-torso IR. Methods: Sprague-Dawley rats (seven per group) were randomized into three groups. The control group underwent midline laparotomy only; the IR group underwent laparotomy and clamping of the infrarenal abdominal aorta for 30 min followed by 2 h of reperfusion; and the third group was pretreated with DEM 6 mmol/kg intraperitoneally 1 h before the IR insult. Results: IR resulted in a significant increase in both microvascular leakage and pulmonary neutrophil infiltration as measured by bronchoalveolar lavage protein concentration and pulmonary myeloperoxidase activity respectively. Pretreatment with DEM significantly attenuated both microvascular leakage and neutrophil infiltration. Conclusion: Preconditioning with DEM protected against IR-induced lung injury. This protective effect raises the possibility of using pro-oxidants to prevent inflammatory injury.

Original languageEnglish
Pages (from-to)482-485
Number of pages4
JournalBritish Journal of Surgery
Volume89
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

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