TY - JOUR
T1 - Differential protein abundance of a basolateral MCT1 transporter in the human gastrointestinal tract
AU - Al-mosauwi, Hashemeya
AU - Ryan, Elizabeth
AU - McGrane, Alison
AU - Riveros-Beltran, Stefanie
AU - Walpole, Caragh
AU - Dempsey, Eugene
AU - Courtney, Danielle
AU - Fearon, Naomi
AU - Winter, Desmond
AU - Baird, Alan
AU - Stewart, Gavin
N1 - Publisher Copyright:
© 2016 International Federation for Cell Biology
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Bacterially derived short chain fatty acids (SCFAs), such as butyrate, are vital in maintaining the symbiotic relationship that exists between humans and their gastrointestinal microbial populations. A key step in this process is the transport of SCFAs across colonic epithelial cells via MCT1 transporters. This study investigated MCT1 protein abundance in various human intestinal tissues. Initial RT-PCR analysis confirmed the expected MCT1 RNA expression pattern of colon > small intestine > stomach. Using surgical resection samples, immunoblot analysis detected higher abundance of a 45 kDa MCT1 protein in colonic tissue compared to ileum tissue (P < 0.001, N = 4, unpaired t-test). Importantly, MCT1 abundance was found to be significantly lower in sigmoid colon compared to ascending colon (P < 0.01, N = 8–11, ANOVA). Finally, immunolocalization studies confirmed MCT1 to be abundant in the basolateral membranes of surface epithelial cells of the ascending, transverse, and descending colon, but significantly less prevalent in the sigmoid colon (P < 0.05, N = 5–21, ANOVA). In conclusion, these data confirm that basolateral MCT1 protein abundance is correlated to levels of bacterially derived SCFAs along the human gastrointestinal tract. These findings highlight the importance of precise tissue location in studies comparing colonic MCT1 abundance between normal and diseased states.
AB - Bacterially derived short chain fatty acids (SCFAs), such as butyrate, are vital in maintaining the symbiotic relationship that exists between humans and their gastrointestinal microbial populations. A key step in this process is the transport of SCFAs across colonic epithelial cells via MCT1 transporters. This study investigated MCT1 protein abundance in various human intestinal tissues. Initial RT-PCR analysis confirmed the expected MCT1 RNA expression pattern of colon > small intestine > stomach. Using surgical resection samples, immunoblot analysis detected higher abundance of a 45 kDa MCT1 protein in colonic tissue compared to ileum tissue (P < 0.001, N = 4, unpaired t-test). Importantly, MCT1 abundance was found to be significantly lower in sigmoid colon compared to ascending colon (P < 0.01, N = 8–11, ANOVA). Finally, immunolocalization studies confirmed MCT1 to be abundant in the basolateral membranes of surface epithelial cells of the ascending, transverse, and descending colon, but significantly less prevalent in the sigmoid colon (P < 0.05, N = 5–21, ANOVA). In conclusion, these data confirm that basolateral MCT1 protein abundance is correlated to levels of bacterially derived SCFAs along the human gastrointestinal tract. These findings highlight the importance of precise tissue location in studies comparing colonic MCT1 abundance between normal and diseased states.
KW - colon
KW - immunolocalization
KW - MCT1
KW - protein abundance
UR - http://www.scopus.com/inward/record.url?scp=84990858970&partnerID=8YFLogxK
U2 - 10.1002/cbin.10684
DO - 10.1002/cbin.10684
M3 - Article
C2 - 27634412
AN - SCOPUS:84990858970
SN - 1065-6995
VL - 40
SP - 1303
EP - 1312
JO - Cell Biology International
JF - Cell Biology International
IS - 12
ER -