TY - JOUR
T1 - Digested galactoglucomannan mitigates oxidative stress in human cells, restores gut bacterial diversity, and provides chemopreventive protection against colon cancer in rats
AU - dos Santos Lima, Amanda
AU - de Oliveira Pedreira, Fernanda Rafaelly
AU - Bento, Nathália Alves
AU - Novaes, Rômulo Dias
AU - dos Santos, Elda Gonçalves
AU - de Almeida Lima, Graziela Domingues
AU - de Almeida, Leonardo Augusto
AU - Belo, Thiago Caetano Andrade
AU - Vieira, Fernando Vitor
AU - Mohammadi, Nima
AU - Kilpeläinen, Petri
AU - Giusti-Paiva, Alexandre
AU - Granato, Daniel
AU - Azevedo, Luciana
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/10
Y1 - 2024/10
N2 - Galactoglucomannan (GGM) is the predominant hemicellulose in coniferous trees, such as Norway spruce, and has been used as a multipurpose emulsifier in the food industry. In vitro digestion with a cellular antioxidant activity assay was performed to determine the bioaccessibility and antioxidant activity of phenolic compounds, and the behaviour of GGM on in vivo experimental assay against induced colon cancer. The results showed that digestion decreased the bioaccessibility and antioxidant capacity of phenolic compounds. Cellular analysis did not support these findings once an antioxidant effect was observed in human cell lines. GGM attenuated the initiation and progression of colon cancer, by reducing the foci of aberrant crypts in rats, and modified the intestinal bacterial microbiota (disrupting the balance between Firmicutes and Bacteroidetes phyla). Thus, GGM provided chemopreventive protection against the development of colon cancer and acted as an intracellular antioxidant agent.
AB - Galactoglucomannan (GGM) is the predominant hemicellulose in coniferous trees, such as Norway spruce, and has been used as a multipurpose emulsifier in the food industry. In vitro digestion with a cellular antioxidant activity assay was performed to determine the bioaccessibility and antioxidant activity of phenolic compounds, and the behaviour of GGM on in vivo experimental assay against induced colon cancer. The results showed that digestion decreased the bioaccessibility and antioxidant capacity of phenolic compounds. Cellular analysis did not support these findings once an antioxidant effect was observed in human cell lines. GGM attenuated the initiation and progression of colon cancer, by reducing the foci of aberrant crypts in rats, and modified the intestinal bacterial microbiota (disrupting the balance between Firmicutes and Bacteroidetes phyla). Thus, GGM provided chemopreventive protection against the development of colon cancer and acted as an intracellular antioxidant agent.
KW - Bioaccessibility
KW - Erythrocytes
KW - Hemicellulose
UR - http://www.scopus.com/inward/record.url?scp=85199148489&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2024.133986
DO - 10.1016/j.ijbiomac.2024.133986
M3 - Article
C2 - 39033896
AN - SCOPUS:85199148489
SN - 0141-8130
VL - 277
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 133986
ER -
