TY - JOUR
T1 - Digestibility and immunoreactivity of soybean β-conglycinin and its deglycosylated form
AU - Amigo-Benavent, Miryam
AU - Clemente, Alfonso
AU - Ferranti, Pasquale
AU - Caira, Simonetta
AU - Del Castillo, M. Dolores
PY - 2011/12/15
Y1 - 2011/12/15
N2 - IgE-mediated allergy to soybean may be connected to an incomplete protein digestion causing an inappropriate immune response in the gut. The aim of this study was to gain insight on the digestibility and immunoreactivity of β-conglycinin and its deglycosylated form. To achieve this goal both proteins were digested mimicking gastrointestinal physiological conditions. Digests were characterised by SDS-PAGE, MALDI-TOF-MS and immunological assays. Data on IgG binding western blot provided key information related to the composition of the digests indicating that whereas deglycosylated β-conglycinin was completely removed by gastrointestinal digestion, α and β subunits of β-conglycinin glycoprotein partially survive the process of digestion. Such differential digestive behaviour might be influenced by glycan moieties, at least to some extent, and should be an additional factor to be considered on its potential allergenicity. Results also support the existence of linear epitopes in the three subunits (α, α′, and β) of both protein forms.
AB - IgE-mediated allergy to soybean may be connected to an incomplete protein digestion causing an inappropriate immune response in the gut. The aim of this study was to gain insight on the digestibility and immunoreactivity of β-conglycinin and its deglycosylated form. To achieve this goal both proteins were digested mimicking gastrointestinal physiological conditions. Digests were characterised by SDS-PAGE, MALDI-TOF-MS and immunological assays. Data on IgG binding western blot provided key information related to the composition of the digests indicating that whereas deglycosylated β-conglycinin was completely removed by gastrointestinal digestion, α and β subunits of β-conglycinin glycoprotein partially survive the process of digestion. Such differential digestive behaviour might be influenced by glycan moieties, at least to some extent, and should be an additional factor to be considered on its potential allergenicity. Results also support the existence of linear epitopes in the three subunits (α, α′, and β) of both protein forms.
KW - β-Conglycinin
KW - Deglycosylation
KW - Glycan moieties
KW - In vitro digestibility
KW - In vitro immunoreactivity
KW - Soybean
UR - http://www.scopus.com/inward/record.url?scp=80051784149&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2011.06.015
DO - 10.1016/j.foodchem.2011.06.015
M3 - Article
AN - SCOPUS:80051784149
SN - 0308-8146
VL - 129
SP - 1598
EP - 1605
JO - Food Chemistry
JF - Food Chemistry
IS - 4
ER -