Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes

Urszula Salaj-Kosla, Sascha Pöller, Wolfgang Schuhmann, Sergey Shleev, Edmond Magner

Research output: Contribution to journalArticlepeer-review

Abstract

The enzyme Trametes hirsuta laccase undergoes direct electron transfer at unmodified nanoporous gold electrodes, displaying a current density of 28μA/cm2. The response indicates that ThLc was immobilised at the surface of the nanopores in a manner which promoted direct electron transfer, in contrast to the absence of a response at unmodified polycrystalline gold electrodes. The bioelectrocatalytic activity of ThLc modified nanoporous gold electrodes was strongly dependent on the presence of halide ions. Fluoride completely inhibited the enzymatic response, whereas in the presence of 150mM Cl-, the current was reduced to 50% of the response in the absence of Cl-. The current increased by 40% when the temperature was increased from 20°C to 37°C. The response is limited by enzymatic and/or enzyme electrode kinetics and is 30% of that observed for ThLc co-immobilised with an osmium redox polymer.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalBioelectrochemistry
Volume91
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Direct electron transfer
  • Laccase
  • Nanoporous gold

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