TY - JOUR
T1 - Effects of Polydopamine Functionalization on Boron Nitride Nanotube Dispersion and Cytocompatibility
AU - Fernandez-Yague, Marc A.
AU - Larrañaga, Aitor
AU - Gladkovskaya, Olga
AU - Stanley, Alanna
AU - Tadayyon, Ghazal
AU - Guo, Yina
AU - Sarasua, Jose Ramon
AU - Tofail, Syed A.M.
AU - Zeugolis, Dimitrios I.
AU - Pandit, Abhay
AU - Biggs, Manus J.
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/10/21
Y1 - 2015/10/21
N2 - Boron nitride nanotubes (BNNTs) have unique physical properties, of value in biomedical applications; however, their dispersion and functionalization represent a critical challenge in their successful employment as biomaterials. In the present study, we report a process for the efficient disentanglement of BNNTs via a dual surfactant/polydopamine (PD) process. High-resolution transmission electron microscopy (HR-TEM) indicated that individual BNNTs become coated with a uniform PD nanocoating, which significantly enhanced dispersion of BNNTs in aqueous solutions. Furthermore, the cytocompatibility of PD-coated BNNTs was assessed in vitro with cultured human osteoblasts (HOBs) at concentrations of 1, 10, and 30 Μg/mL and over three time-points (24, 48, and 72 h). In this study it was demonstrated that PD-functionalized BNNTs become individually localized within the cytoplasm by endosomal escape and that concentrations of up to 30 Μg/mL of PD-BNNTs were cytocompatible in HOBs cells following 72 h of exposure.
AB - Boron nitride nanotubes (BNNTs) have unique physical properties, of value in biomedical applications; however, their dispersion and functionalization represent a critical challenge in their successful employment as biomaterials. In the present study, we report a process for the efficient disentanglement of BNNTs via a dual surfactant/polydopamine (PD) process. High-resolution transmission electron microscopy (HR-TEM) indicated that individual BNNTs become coated with a uniform PD nanocoating, which significantly enhanced dispersion of BNNTs in aqueous solutions. Furthermore, the cytocompatibility of PD-coated BNNTs was assessed in vitro with cultured human osteoblasts (HOBs) at concentrations of 1, 10, and 30 Μg/mL and over three time-points (24, 48, and 72 h). In this study it was demonstrated that PD-functionalized BNNTs become individually localized within the cytoplasm by endosomal escape and that concentrations of up to 30 Μg/mL of PD-BNNTs were cytocompatible in HOBs cells following 72 h of exposure.
UR - https://www.scopus.com/pages/publications/84945560709
U2 - 10.1021/acs.bioconjchem.5b00257
DO - 10.1021/acs.bioconjchem.5b00257
M3 - Article
C2 - 26282841
AN - SCOPUS:84945560709
SN - 1043-1802
VL - 26
SP - 2025
EP - 2037
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 10
ER -